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dc.contributor.authorTownson, David H.es_ES
dc.contributor.authorPutnam, Amanda N.es_ES
dc.contributor.authorSullivan, Brian T.-
dc.contributor.authorGuo, Lankai-
dc.contributor.authorIrving-Rodgers, Helen F.-
dc.date.accessioned2015-09-29T07:17:44Z-
dc.date.available2015-09-29T07:17:44Z-
dc.date.issued2010-
dc.identifier.issn0213-3911es_ES
dc.identifier.urihttp://hdl.handle.net/10201/46318-
dc.description.abstractApoptosis is a mechanism of cell elimination during follicular atresia and luteal regression. Recent evidence suggests sensitivity to apoptosis in some cell types is partly dependent upon cytokeratin-containing intermediate filaments. Specifically, cytokeratin 8/18 (CK8/18) filaments are thought to impart resistance to apoptosis. Here, cytokeratin filament expression within bovine ovarian follicles and corpora lutea (CL) was characterized and the potential relationship between cellspecific CK8/18 expression and apoptosis explored. Immunoprecipitation and western blot analysis confirmed CK8 associates with CK18 to form CK8/18 heterodimeric filaments within bovine ovarian cells. Immunostaining revealed populations of CK18-positive (CK18+) cells in healthy growing follicles that increased in postovulatory follicles. Atretic follicles at all stages of atresia also contained some CK18+ cells. However, no CK18+ cells were detected in primordial or primary follicles. In CL, developing CL contained a higher proportion of CK18+ cells (~35%, range 30-70%) than mature CL (~16%) and regressing CL (~5%; P<0.05, n = 3-5 CL/stage), suggesting CK8/18 filament expression diminishes over time, as luteal cells become more susceptible to apoptosis. Dual-fluorescence labeling for CK18 and a cell death marker (TUNEL labeling) confirmed this view, demonstrating less death of CK18+ than CK18- luteal cells throughout the estrous cycle (P<0.05). The results indicate differential expression of CK8/18 filaments occurs in cells of bovine ovarian follicles and CL throughout the estrous cycle. The prevalence and cell-specific pattern of cytokeratin expression in these structures is consistent with the concept these filaments might impart resistance to apoptosis in ovarian cells as is seen in other cell types.es_ES
dc.formatapplication/pdfes_ES
dc.format.extent12es_ES
dc.languageenges_ES
dc.publisherMurcia : F. Hernándezes_ES
dc.relation.ispartofHistology and histopathologyes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectOvaryes_ES
dc.subjectApoptosises_ES
dc.subject.other576 - Biología celular y subcelular. Citologíaes_ES
dc.titleExpression and distribution of cytokeratin 8-18 intermediate filaments in bovine antral follicles and corpus luteum: An intrinsic mechanism of resistance to apoptosis?es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
Aparece en las colecciones:Vol.25, nº7 (2010)



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