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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Townson, David H. | es |
dc.contributor.author | Putnam, Amanda N. | es |
dc.contributor.author | Sullivan, Brian T. | - |
dc.contributor.author | Guo, Lankai | - |
dc.contributor.author | Irving-Rodgers, Helen F. | - |
dc.date.accessioned | 2015-09-29T07:17:44Z | - |
dc.date.available | 2015-09-29T07:17:44Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0213-3911 | es |
dc.identifier.uri | http://hdl.handle.net/10201/46318 | - |
dc.description.abstract | Apoptosis is a mechanism of cell elimination during follicular atresia and luteal regression. Recent evidence suggests sensitivity to apoptosis in some cell types is partly dependent upon cytokeratin-containing intermediate filaments. Specifically, cytokeratin 8/18 (CK8/18) filaments are thought to impart resistance to apoptosis. Here, cytokeratin filament expression within bovine ovarian follicles and corpora lutea (CL) was characterized and the potential relationship between cellspecific CK8/18 expression and apoptosis explored. Immunoprecipitation and western blot analysis confirmed CK8 associates with CK18 to form CK8/18 heterodimeric filaments within bovine ovarian cells. Immunostaining revealed populations of CK18-positive (CK18+) cells in healthy growing follicles that increased in postovulatory follicles. Atretic follicles at all stages of atresia also contained some CK18+ cells. However, no CK18+ cells were detected in primordial or primary follicles. In CL, developing CL contained a higher proportion of CK18+ cells (~35%, range 30-70%) than mature CL (~16%) and regressing CL (~5%; P<0.05, n = 3-5 CL/stage), suggesting CK8/18 filament expression diminishes over time, as luteal cells become more susceptible to apoptosis. Dual-fluorescence labeling for CK18 and a cell death marker (TUNEL labeling) confirmed this view, demonstrating less death of CK18+ than CK18- luteal cells throughout the estrous cycle (P<0.05). The results indicate differential expression of CK8/18 filaments occurs in cells of bovine ovarian follicles and CL throughout the estrous cycle. The prevalence and cell-specific pattern of cytokeratin expression in these structures is consistent with the concept these filaments might impart resistance to apoptosis in ovarian cells as is seen in other cell types. | es |
dc.format | application/pdf | es |
dc.format.extent | 12 | es |
dc.language | eng | es |
dc.publisher | Murcia : F. Hernández | es |
dc.relation.ispartof | Histology and histopathology | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Ovary | es |
dc.subject | Apoptosis | es |
dc.subject.other | 576 - Biología celular y subcelular. Citología | es |
dc.title | Expression and distribution of cytokeratin 8-18 intermediate filaments in bovine antral follicles and corpus luteum: An intrinsic mechanism of resistance to apoptosis? | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.25, nº7 (2010) |
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Expression and distribution of cytokeratin 818 intermediate filaments in bovine antral follicles and corpus luteum....pdf | 2,64 MB | Adobe PDF | Visualizar/Abrir |
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