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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Martín-Orozco Santiago, Elena | es |
dc.contributor.author | Chicano Saura, Antonio | es |
dc.contributor.author | Ruiz Alcaraz, Antonio José | - |
dc.contributor.author | Lizana, Ana | - |
dc.date.accessioned | 2015-09-29T07:17:43Z | - |
dc.date.available | 2015-09-29T07:17:43Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0213-3911 | es |
dc.identifier.uri | http://hdl.handle.net/10201/46317 | - |
dc.description.abstract | Aims: In this work we aimed to investigate the expression of TLR9 protein in the murine hepatocyte cell line TIB-73, compared to macrophage-like J774 cells, by Western blot analysis, and the role played by ERK 1/2 MAP kinase in the intracellular signals triggered by stimulation with CpG and non-CpG phosphodiester-ODN, and their more stable phosphorothioate-modified analogues. Results: TIB-73 hepatocytes express TLR9 protein. CpG and non-CpG ODN stimulation activated ERK 1/2 MAPK signal pathway in both hepatocytes and J774 murine macrophages. As expected, their phosphorothioate-CpG and non-CpG ODN analogues induced higher levels of ERK1/2 phosphorylation in TIB-73 cells, even higher than that induced in J774 cells under the same conditions. Phosphorylation of ERK 1/2 induced by synthetic ODN is dose-response dependent, being maximal at 100 µg/ml. Pretreatment of hepatocytes with an inhibitor of MEK-1 abrogated phosphorylation of ERK1/2 kinase. Conclusions: TIB-73 hepatocytes constitutively express TLR9 and respond to synthetic ODN stimulation through a high ERK1/2 phosphorylation independent of CpG motifs. Slight differences were found on ERK1/2 activation when using phosphorothioate versus phosphodiester oligonucleotides. | es |
dc.format | application/pdf | es |
dc.format.extent | 10 | es |
dc.language | eng | es |
dc.publisher | Murcia : F. Hernández | es |
dc.relation.ispartof | Histology and histopathology | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Hepatocytes | es |
dc.subject.other | 577 - Bioquímica. Biología molecular. Biofísica | es |
dc.title | Synthetic oligodeoxynucleotides induce MAP kinases activation in murine TIB-73 hepatocytes | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.25, nº7 (2010) |
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Synthetic oligodeoxynucleotides induce MAP kinases activation in murine TIB73 hepatocytes.pdf | 1,18 MB | Adobe PDF | Visualizar/Abrir |
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