Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10201/46317

Registro completo de metadatos
Campo DCValorLengua/Idioma
dc.contributor.authorMartín-Orozco Santiago, Elenaes
dc.contributor.authorChicano Saura, Antonioes
dc.contributor.authorRuiz Alcaraz, Antonio José-
dc.contributor.authorLizana, Ana-
dc.date.accessioned2015-09-29T07:17:43Z-
dc.date.available2015-09-29T07:17:43Z-
dc.date.issued2010-
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/46317-
dc.description.abstractAims: In this work we aimed to investigate the expression of TLR9 protein in the murine hepatocyte cell line TIB-73, compared to macrophage-like J774 cells, by Western blot analysis, and the role played by ERK 1/2 MAP kinase in the intracellular signals triggered by stimulation with CpG and non-CpG phosphodiester-ODN, and their more stable phosphorothioate-modified analogues. Results: TIB-73 hepatocytes express TLR9 protein. CpG and non-CpG ODN stimulation activated ERK 1/2 MAPK signal pathway in both hepatocytes and J774 murine macrophages. As expected, their phosphorothioate-CpG and non-CpG ODN analogues induced higher levels of ERK1/2 phosphorylation in TIB-73 cells, even higher than that induced in J774 cells under the same conditions. Phosphorylation of ERK 1/2 induced by synthetic ODN is dose-response dependent, being maximal at 100 µg/ml. Pretreatment of hepatocytes with an inhibitor of MEK-1 abrogated phosphorylation of ERK1/2 kinase. Conclusions: TIB-73 hepatocytes constitutively express TLR9 and respond to synthetic ODN stimulation through a high ERK1/2 phosphorylation independent of CpG motifs. Slight differences were found on ERK1/2 activation when using phosphorothioate versus phosphodiester oligonucleotides.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectHepatocyteses
dc.subject.other577 - Bioquímica. Biología molecular. Biofísicaes
dc.titleSynthetic oligodeoxynucleotides induce MAP kinases activation in murine TIB-73 hepatocyteses
dc.typeinfo:eu-repo/semantics/articlees
Aparece en las colecciones:Vol.25, nº7 (2010)

Ficheros en este ítem:
Fichero Descripción TamañoFormato 
Synthetic oligodeoxynucleotides induce MAP kinases activation in murine TIB73 hepatocytes.pdf1,18 MBAdobe PDFVista previa
Visualizar/Abrir


Los ítems de Digitum están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.