Please use this identifier to cite or link to this item: http://hdl.handle.net/10201/46306

Full metadata record
DC FieldValueLanguage
dc.contributor.authorBelhocine, M.es
dc.contributor.authorGernigon-Spychalowicz, T.es
dc.contributor.authorJacob, M.P.-
dc.contributor.authorBenazzoug, Y.-
dc.contributor.authorExbrayat, Jean Marie-
dc.date.accessioned2015-09-29T07:17:01Z-
dc.date.available2015-09-29T07:17:01Z-
dc.date.issued2010-
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/46306-
dc.description.abstractAn immunohistochemical study of matrix metalloproteinases (MMP-2 and MMP-9) or gelatinase (gelatinase A and gelatinase B) was performed on the seminal vesicles and ventral prostate of the Libyan jird (Meriones libycus) collected in the Beni-Abbes area during breeding period (spring and early summer), during resting phase (late summer, autumn, winter) and from castrated animals in the spring. The work was done using the indirect immunohistochemistry protocol by amplification with streptavidin-biotin-peroxidase and AEC as chromogen. In the seminal vesicles, during the breeding period, an important immunohistochemical signal of MMP-2 and MMP-9 was observed in epithelial cells and smooth muscle cells (SMC) without any immunoexpression in the extracellular matrix (ECM) and secretion. During resting phase and in thirty days castrated Meriones libycus, the MMP-2 and MMP-9 immunoexpression was weak in the epithelial cells and persisted with the same intensity in the SMC. The ECM, with no immunostaining in active season, showed a pronounced immunoresponse of both the two gelatinase. Three days after castration, the MMP-9 immunohistochemical reaction in epithelial cells and SMC was as intense as during active season. A prolonged castration of 50 and 90 days resulted in the maintenance of the MMP-9 immunostaining in epithelial cells and SMC and its disappearance from the ECM, suggesting a slow process of regression. During the breeding period, in the ventral prostate, MMP-2 immunostaining was more important in the SMC than in epithelial cells. The MMP-9 immunoexpression pattern was the opposite, the epithelial cells showed a higher immunoreaction than SMC. ECM and secretion lacked MMP-2 and MMP-9 immunostaining. The ventral prostate lumen contained a granular secretion without any gelatinase immunolabelling and was hollowed by empty circular forms reflecting the disappearance of the product in these areas. Part of the secretion showed a positive MMP-2 and MMP-9 immunoreaction. The latter was subsequently filled and seemed involved in the progression of the secretion in the tubules, preventing their filling. In resting phase and in animals castrated since thirty days, the immunoreactivity of both the two gelatinases was maintained in the epithelial cells and in the SMC, and was absent in the ECM. The gelatinases are involved in the seasonal reproductive cycle of Meriones libycuses
dc.formatapplication/pdfes
dc.format.extent18es
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectReproductiones
dc.subjectSeminal vesiclees
dc.subjectProstate-
dc.subject.other57 - Biologíaes
dc.titleImmunoexpression of gelatinase (MMP-2 and MMP-9) in the seminal vesicles and ventral prostate of Libyan jird (Meriones libycus) during the seasonal cycle of reproductiones
dc.typeinfo:eu-repo/semantics/articlees
Appears in Collections:Vol.25, nº5 (2010)



Items in Digitum are protected by copyright, with all rights reserved, unless otherwise indicated.