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Título: | Carbonic anhydrase II is secreted from bovine parotid glands |
Fecha de publicación: | 2010 |
Editorial: | Murcia : F. Hernández |
ISSN: | 0213-3911 |
Materias relacionadas: | 636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticos |
Palabras clave: | Parotid gland Cattle |
Resumen: | Besides carbonic anhydrase VI (CA-VI),CA-II is suggested to be a second secreted isoenzyme inruminant saliva. Therefore, the aim of the present studywas to investigate the expression of salivary CA-II inbovine parotid glands at the protein level. Moreover, weintended to identify the cells which secrete the enzymeinto the saliva. Two commercially available CA-IIspecific antibodies were tested for use inimmunohistochemistry on frozen sections of bovineparotid tissue. Intense positive staining for CA-II wasfound in luminal duct cells and for the first time alsoinside the duct lumen, clearly demonstrating theexpression and secretion of salivary CA-II in bovineparotid glands. The presence of CA-II protein wasverified for parotid tissue and whole saliva usingimmunoblot analysis. Both salivary CA-II and CA-VIare highly active in supplying the alimentary tract withbicarbonate. It is suggested that a decrease in theexpression of either one of these enzymes might severelydisturb digestion and/or increase susceptibility toacidosis in ruminants. |
Autor/es principal/es: | Mau, M. Kaiser, T.M. Südekum, K.-H. |
Forma parte de: | Histology and histopathology |
URI: | http://hdl.handle.net/10201/42592 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 9 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol.25, nº3 (2010) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Carbonic anhydrase II is secreted from bovine parotid glands.pdf | 5,61 MB | Adobe PDF | Visualizar/Abrir |
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