Please use this identifier to cite or link to this item: http://hdl.handle.net/10201/36021

Title: Cytochemical and biochemical evidences for a complex tridimensional structure of the hamster zona pellucida
Issue Date: 2009
Publisher: Murcia : F. Hernández
ISSN: 0213-3911
Related subjects: 576 - Biología celular y subcelular. Citología
Keywords: Cytochemistry
Hamster
Abstract: Zona pellucida (ZP) is an extracellular matrix that surrounds eggs and pre-implantation embryos and is required for in vivo fertility. A key event in successful fertilization is sperm binding to the surface of the ZP. It has been previously described that the hamster sperm binds mainly the outer region of the ZP which corresponds to the porous region in contact with the cumulus cells. Using ultrastructural cytochemistry approaches with an antibody developed against porcine ZP, this study shows that the pig ZP shares epitopes with some rodent species like hamster, rat and mouse. In the hamster, these epitopes are located mainly in the outer region of the ZP of preovulatory and ovulated oocytes. By means of biochemical approaches it was demonstrated that 1) the antibody is specific for the native hamster ZP3, 2) four different bands with a molecular weight of 67, 60, 48 and 38 kDa after Nlinked deglycosylation suggesting that the hamster ZP is formed by four proteins, and 3) the different composition observed in the outer region of the hamster ZP could be due to a specific supramolecular structure that makes some epitopes accessible for the antibodies. In summary, this study provides evidence that the different composition observed in the different regions of the ZP is mediated by a different organization of the components of the ZP produced during the oocyte maturation. This different organization could be responsible for the different sperm binding affinity observed for sperm to the outer region versus the inner region of the ZP.
Primary author: Jiménez Movilla, María
Martínez Alonso, Emma
Castells Mora, María Teresa
Izquierdo Rico, María José
Saavedra Leos, María Dolores
Gutiérrez Gallego, Ricardo
Fayrer Hosken, Richard
Ballesta, José
Avilés Sánchez, Manuel
Faculty / Departments / Services: Biología Celular
Published in: Histology and histopathology
URI: http://hdl.handle.net/10201/36021
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 11
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Vol.24, nº5 (2009)



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