Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10201/29868

Título: Morphological effects of oestradiol-17ß‚ and selective oestrogen receptor a and ß agonists on luteinising hormone-secreting
Fecha de publicación: 2008
Editorial: Murcia : F. Hernández
ISSN: 0213-3911
Materias relacionadas: 61 - Medicina
Palabras clave: Gonadotroph morphology
Selective ER agonists
Resumen: Summary. To investigate the role played by the different rat gonadotroph oestrogen receptor (ER) pools in the effects of oestradiol-17ß (E2) on gonadectomy cells, two-week ovariectomised (OVX) rats were used. The basic experimental group of rats was injected with 3 mg of the selective ER modulator tamoxifen (TX) on days 15-20 after OVX. Groups of TX-treated OVX rats were additionally injected on days 18-20 after OVX with 10 µg oestradiol benzoate (EB), 1 mg of the selective ERα agonist propylpyrazole triol (PPT), or 1 mg of the selective ERß diarylpropionitrile (DPN). Negative and positive control groups were OVX rats injected over six days after OVX with 0.2 ml oil and EB, respectively. On day 21 after OVX, anterior pituitary glands were dissected out and divided into halves. One hemipituitary was processed for light microscopy and immunocytochemistry for ßLH subunit and progesterone receptor (PR), and the other hemipituitary for ultrastructural evaluation. Results showed that: gonadotrophs were the only pituitary cell type expressing PR; treatment with TX alone shrunk gonadectomy cells and induced both reorganization of membrane-enclosed intracellular organelles and PR expression, and treatment with DPN or EB, but not PPT, reduced the agonistic morphological effects of TX. Considering that TX activates nuclear ERα, the results indicate that activation of nuclear ERα is determinant for the reversal effects of E2 on gonadotrope morphology and PR expression, and the simultaneous activation of ERß modulates the action of ERα in an inhibitory fashion.
Autor/es principal/es: Garrido-Gracia, José C.
Gordon, Ana
Aguilar, Rafaela
Monterde, J. G.
Blanco, A.
Martín de las Mulas, J.
Sánchez-Criado, J.E.
Forma parte de: Histology and histopathology
URI: http://hdl.handle.net/10201/29868
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 11
Derechos: info:eu-repo/semantics/openAccess
Aparece en las colecciones:Vol.23,nº12 (2008)



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