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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Burns, Jorge S. | es |
dc.contributor.author | Abdallah, Basem M. | es |
dc.contributor.author | Schröder, Henrik E. | - |
dc.contributor.author | Kassem, Moustapha | - |
dc.date.accessioned | 2013-01-22T10:03:54Z | - |
dc.date.available | 2013-01-22T10:03:54Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0213-3911 | es |
dc.identifier.uri | http://hdl.handle.net/10201/29835 | - |
dc.description.abstract | Sarcomas display varied degrees of karyotypic abnormality, vascularity and mesenchymal with a branching periodic acid Schiff reaction pattern. Such clone-specific differences in host vascular response provide novel models to explore interactions between mesenchymal stem and endothelial cells. Despite the lack of a characteristic chromosomal translocation, the histomorphology, biomarkers and oncogenic changes were similar to those prevalent for Ewing’s sarcomas. The phenotype and ontogenesis of hMSC-TERT20 tumors was consistent with the hypothesis that sarcomas may arise from hMSC, providing a unique diploid model for exploring human sarcoma biology. differentiation. We have reported that a strain of telomerized adult human bone marrow mesenchymal stem cells (hMSC-TERT20) spontaneously evolved a tumorigenic phenotype after long-term continuous culture. We asked to what extent our hMSC-TERT20 derived tumors reflected events found in human sarcomas using routine histopathological procedures. Early versus late passage hMSC-TERT20 cultures persistently expressed mesenchymal lineage proteins e.g. CD105, CD44, CD99 and vimentin. However, late passage cultures, showed increased immunohistochemical staining for CyclinD1 and p21WAF1/Cip1, whereas p27Kip1 staining was reduced. Notably, spectral karyotyping showed that tumorigenic hMSC-TERT20 cells retained a normal diploid karyotype, with no detectable chromosome abnormalities. Consistent with the bone-forming potential of early passage hMSC-TERT20 cells, tumors derived from late passage cells expressed early biomarkers of osteogenesis. However, hMSC-TERT20 cells were heterogeneous for alpha smooth muscle actin (ASMA) expression and one out of six hMSC-TERT20 derived single cell clones was strongly ASMA positive. Tumors from this ASMA+ clone had distinctive vascular qualities with hot spots of high CD34+ murine endothelial cell density, together with CD34- regions | es |
dc.format | application/pdf | es |
dc.format.extent | 12 | es |
dc.language | eng | es |
dc.publisher | Murcia : F. Hernández | es |
dc.relation.ispartof | Histology and histopathology | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Cell culture | es |
dc.subject | Angiogenesis | es |
dc.subject.other | 61 - Medicina | es |
dc.title | The histopathology of a human mesenchymal stem cell experimental tumor model: support for an hMSC origin for Ewing`s sarcoma? | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.23,nº10 (2008) |
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The histopathology of a human mesenchymal stem cell experimental tumor model support for an hMSC origin for Ewing s sarcoma.pdf | 13,89 MB | Adobe PDF | Visualizar/Abrir |
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