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http://hdl.handle.net/10201/29799
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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Desantis, S. | es |
dc.contributor.author | Albrizio, M. | es |
dc.contributor.author | Ventriglia, G. | - |
dc.contributor.author | Deflorio, M. | - |
dc.contributor.author | Guaricci, A.C. | - |
dc.contributor.author | Minoia, R. | - |
dc.contributor.author | De Metrio, G. | - |
dc.date.accessioned | 2013-01-22T10:01:02Z | - |
dc.date.available | 2013-01-22T10:01:02Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0213-3911 | es |
dc.identifier.uri | http://hdl.handle.net/10201/29799 | - |
dc.description.abstract | The presence of the μ-opioid receptor and the type of glycosylation in the third extra-cellular loop of this receptor was investigated in the isthmus of mare oviduct during oestrus by means of immunoblotting and immunohistochemistry combined with enzymatic (Nglycosidase F and O-glycosidase) and chemical (ßelimination) treatments. Immunoblotting analysis showed that the μ -opioid receptor consists of two peptides with molecular weights of around 65 and 50 kDa. After N-deglycosylation with N-glycosidase F an additional immunoreactive peptide was observed at around 30 KDa. The cleavage of O-glycans by Oglycosidase failed in immunoblotting as well as in immunohistochemistry investigations, revealing that the third extra-cellular loop of the μ -opioid receptor expressed in mare isthmus oviduct contains some modifications of the Galß(1-3)GalNAc core binding to serine or threonine. Immunohistochemistry revealed the μ-opioid receptor in the mucosal epithelium, some stromal cells, muscle cells and blood vessels. In ciliated cells the μ-opioid receptor showed N-linked glycans, since the immunoreactivity was abolished after Nglycosidase F treatment, whereas it was preserved in the apical region after ß-elimination. Most non-ciliated cells expressed the μ-opioid receptor with both N- and Olinked oligosaccharides, as revealed by the abolition of immunostaining after N-glycosidase F and ßelimination. Stromal cells, endothelial and muscle cells of blood vessels expressed the μ -opioid receptor containing both N- and O-linked oligosaccharides. Myosalpinx myocytes expressed the μ-opioid receptor with O-linked oligosaccharides. The immunopositive myocytes formed a circular coat in the intrinsic musculature, whereas they were arranged in some isolated, oblique bundles in the extrinsic musculature. In conclusion, the μ-opioid receptor could have a role in the production and the movement of isthmus lumen content that contributes to ensuring the effective condition of the sperm in the mare oviduct. | es |
dc.format | application/pdf | es |
dc.format.extent | 10 | es |
dc.language | eng | es |
dc.publisher | Murcia : F. Hernández | es |
dc.relation.ispartof | Histology and histopathology | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Oviduct | es |
dc.subject | Glycosylation | es |
dc.subject.other | 636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticos | es |
dc.title | The presence of the u-opioid receptor in the isthmus of mare oviduct | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.23, nº5 (2008) |
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Fichero | Descripción | Tamaño | Formato | |
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The presence of the u-opioid receptor in the isthmus of mare oviduct.pdf | 4,44 MB | Adobe PDF | ![]() Visualizar/Abrir |
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