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Título: The role of nitric oxide in remodeling of capillary network in rat interscapular brown adipose tissue after long-term cold acclimation
Fecha de publicación: 2008
Editorial: Murcia : F. Hernández
ISSN: 0213-3911
Materias relacionadas: 59 - Zoología
Palabras clave: Capillary network
Nitric oxide
Resumen: Cold exposure has been shown to increase blood flow in interscapular brown adipose tissue (IBAT). The aim of the present study was to evaluate the role of the L-arginine-nitric oxide (•NO) pathway on IBAT capillary network remodeling and its possible correlation with superoxide anion radical (O2 •–). In the rats that received L-arginine (2.25%) or NG-nitro-L-arginine methyl ester (L-NAME, 0.01%) as a drinking liquid and maintained at room (22±1°C) or low (4±1°C) temperature for 45 days, IBAT capillaries were analyzed by stereology and observed by light and electron microscopy. Additionally, endothelial •NO synthase (eNOS) expression, nitrotyrosine immunoreactivity and both copper zinc superoxide dismutase (CuZnSOD) enzyme activity and immunohistochemical localization were examined. Stereological analyses of IBAT show that the capillary volume density, as well as capillary-tobrown adipocytes ratio, are increased in cold. L-arginine treatment increases, while L-NAME decreases both parameters, compared to respective controls. Those changes were accompanied by capillary dilatation observed by light and electron microscopy. The activity of CuZnSOD is lower in control cold-acclimated rats, as well as in both L-arginine-treated groups, when compared to control animals acclimated to room temperature. L-NAME treatment attenuates the effects both of cold and L-arginine on CuZnSOD and increases immunopositivity for CuZnSOD in room temperatureacclimated rats. Our results show that •NO induces remodeling of the IBAT capillary network by angiogenesis, and presumably that interaction with O2 •– has a role in that modulation. The increased eNOS expression accompanied by an increased nitrotyrosine immunoreaction observed in both L-arginine-treated groups compared to corresponding controls strengthens this hypothesis.
Autor/es principal/es: Korac, A.
Buzadzic, B.
Petrovic, V.
Vasilijevic, A.
Jankovic, A.
Micunovic, K.
Korac, B.
Publicado en: Histology and histopathology
URI: http://hdl.handle.net/10201/29796
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 10
Derechos: info:eu-repo/semantics/openAccess
Aparece en las colecciones:Vol.23, nº4 (2008)

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