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Título: | Fibroblast remodeling of adsorbed collagen type IV is altered in contact with cancer cells |
Fecha de publicación: | 2008 |
Editorial: | Murcia : F. Hernández |
ISSN: | 0213-3911 |
Materias relacionadas: | 616 - Patología. Medicina clínica. Oncología |
Palabras clave: | Fibroblasts Cancer cells |
Resumen: | A series of co-culture experiments between fibroblasts and H-460 human lung carcinoma cells were performed to learn more about the fate of adsorbed type IV collagen (Coll IV). Fibroblasts were able to spatially rearrange Coll IV in a specific linear pattern, similar but not identical to the fibronectin (FN) fibrils. Coll IV partly co-aligns with fibroblast actin cytoskeleton and transiently co-localize with FN, as well as with ß1 and a2 integrin clusters, suggesting a cell-dependent process. We further found that this Coll IV reorganization is suppressed in contact with H460 cells. Zymography revealed strongly elevated MMP-2 activity in supernatants of co-cultures, but no activity when fibroblasts or cancer cells were cultured alone. Thus, we provide evidence that reorganization of substrate associated Coll IV is a useful morphological approach for in vitro studies on matrix remodeling activity during tumorigenesis. |
Autor/es principal/es: | Maneva-Radicheva, L. Ebert, U. Dimoudis, N. Altankov, G. |
Forma parte de: | Histology and histopathology |
URI: | http://hdl.handle.net/10201/29709 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 10 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol.23, nº7 (2008) |
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Fibroblast remodeling of adsorbed collagen type IV is altered in contact with cancer cells.pdf | 5,13 MB | Adobe PDF | Visualizar/Abrir |
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