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Título: Isolation of high quality protein samples from punches of formalin fixed and paraffin embedded tissue blocks
Fecha de publicación: 2008
Editorial: Murcia : F. Hernández
ISSN: 0213-3911
Materias relacionadas: 616.5 - Piel. Dermatología clínica
Palabras clave: Proteins
Insolation
Resumen: In general, it is believed that the extraction of proteins from formalin-fixed paraffin embedded samples is not feasible. However, recently a new technique was developed, presenting the extraction of non-degraded, full length proteins from formalin fixed tissues, usable for western blotting and protein arrays. In the study presented here, we applied this technique to punch biopsies of formalin fixed tissues embedded in paraffin to reduce heterogeneity of the tissue represented in sections, and to ensure analysing mainly defined cellular material. Successful extraction was achieved even from very small samples (0.7 mm3). Additionally, we were able to detect highly glycosylated proteins and protein modification, such as phosphorylation. Interestingly, with this technique it is feasible to extract high quality proteins from 14 year old samples. In summary, the new technique makes a great pool of material now usable for molecular analysis with high throughput tools.
Autor/es principal/es: Kroll, J.
Becker, K.F.
Kuphal, S.
Hein, R.
Hofstädter, F.
Bosserhoff, A.K.
Publicado en: Histology and histopathology
URI: http://hdl.handle.net/10201/29688
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 5
Derechos: info:eu-repo/semantics/openAccess
Aparece en las colecciones:Vol.23, nº4 (2008)



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