Por favor, use este identificador para citar o enlazar este ítem:
http://hdl.handle.net/10201/29677
Twittear
Título: | Abnormal collagen deposition in synovia after collagen type V immunization in rabbits |
Fecha de publicación: | 2008 |
Editorial: | Murcia : F. Hernández |
ISSN: | 0213-3911 |
Materias relacionadas: | 616.7 - Patología de los órganos de la locomoción. Sistema locomotor y esquelético |
Palabras clave: | Collagen Fibrosis |
Resumen: | Sinovitis in Scleroderma (SSc) is rare, usually aggressive and fully resembles rheumatoid arthritis. Experimental models of SSc have been used in an attempt to understand its pathogenesis. Previous studies done in our laboratory had already revealed the presence of a synovial remodeling process in rabbits immunized with collagen V. To validate the importance of collagen type V and to explore the quantitative relationship between this factor and synovia remodeling as well as the relationship between collagen type V and other collagens, we studied the synovial tissue in immunized rabbits. Rabbits (N=10) were immunized with collagen V plus Freund’s adjuvant and compared with animals inoculated with adjuvant only (N=10). Synovial tissues were submitted to histological analysis, immunolocalization to collagen I, III and V and biochemical analysis by eletrophoresis, immunoblot and densitometric method. The synovial tissue presented an intense remodeling process with deposits of collagen types I, III and V after 75 and 120 days of immunization, mainly distributed around the vessels and interstitium of synovial extracellular matrix. Densitometric analysis confirmed the increased synthesis of collagen I, III and V chains (407.69±80.31; 24.46±2.58; 70.51±7.66, respectively) in immunized rabbits when compared with animals from control group (164.91±15.67; 12.89±1.05; 32±3.57) (p<0.0001). We conclude that synovial remodeling observed in the experimental model can reflect the articular compromise present in patients with scleroderma. Certainly, this experimental model induced by collagen V immunization will bring new insights in to pathogenic mechanisms and allow the testing of new therapeutic strategies to ameliorate the prognosis for scleroderma patients. |
Autor/es principal/es: | Tsuzuki Ichicawa Ogido, Luciana Walcy Rosolia, Teodoro Pereira Velosa, Ana Paula De Oliveira, Cristiane Carla Roger Parra, Edwin Capelozzi, Vera Luiza Hajime Yoshinari, Natalino |
Forma parte de: | Histology and histopathology |
URI: | http://hdl.handle.net/10201/29677 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 7 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol.23, nº3 (2008) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Abnormal collagen deposition in synovia after collagen type V immunization in rabbits.pdf | 957,03 kB | Adobe PDF | Visualizar/Abrir |
Los ítems de Digitum están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.