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Title: Apoptosis in peripheral neuroblastic tumors. Immunohistochemical expression of bcl-2 and p53 is related to DNA fragmentation
Issue Date: 2007
Publisher: Murcia: F. Hernández
ISSN: 0213-3911
Related subjects: 616.8 - Neurología. Neuropatología. Sistema nervioso
Keywords: Neuroblastoma
Biochemical apoptosis
Abstract: We examined 111 cases of neuroblastoma (NB), searching for how NB relates to apoptotic control and other prognostic factors. Immunohistochemistry using avidin-biotin-peroxidase was carried out for bcl-2 and p53 proteins. Apoptosis was analyzed by in situ detection of chromosomal breakdown. DNA ladders were detected by electrophoresis and amplification of MYCN was carried out by PCR and Southern blot. Statistical analyses were performed with Pearson’s c2 and Kruskal-Wallis tests and Cox’s regression. We found expression of bcl-2 protein mainly in cases of neuroblastoma without differentiation and in stages 3 and 4. Expression of p53 protein showed a correlation with bcl-2 and the apoptotic phenomenon; apoptosis was found mainly in favorable cases. Multivariate analysis showed bcl-2 protein expression to be the most independent risk factor. The study of apoptosis could be important for the design of therapies to treat neuroblastoma.
Primary author: Mejía, C.
Navarro, S.
Llombart-Bosch, A.
Published in: Histology and histopathology, vol. 22, nº12 (2007)
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 6
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Vol.22, nº12 (2007)

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