Please use this identifier to cite or link to this item: http://hdl.handle.net/10201/27654

Full metadata record
DC FieldValueLanguage
dc.contributor.authorOhno, N.es
dc.contributor.authorTerada, N.es
dc.contributor.authorSaitoh, S.-
dc.contributor.authorZhou, H.-
dc.contributor.authorFuji, Y.-
dc.contributor.authorOhno, S.-
dc.date.accessioned2012-05-21T12:11:23Z-
dc.date.available2012-05-21T12:11:23Z-
dc.date.issued2007-
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/27654-
dc.description.abstractVarious microscopic methods have been used to analyze the morphology and molecular distribution of cells and tissues. Using conventional procedures, however, ischemic or anoxic artifacts are inevitably caused by tissue-resection or perfusion-fixation. The in vivo cryotechnique (IVCT) was developed to overcome these problems, and was found to be useful with light microscopy for analyses of the distribution of watersoluble molecules without anoxic effects at high time resolution. But there are limitations to the application of IVCT, such as exposure of target organs of living small animals and immunoreactivity of lipid-soluble molecules owing to freeze-substitution with acetone. Recently, a new cryotechnique called “cryobiopsy” has been developed, which enables one to obtain tissue specimens of large animals including humans without ischemia or anoxia, and has almost the same technical advantages as IVCT. Both IVCT and cryobiopsy complement other live-imaging techniques, and are useful for not only the morphological observation of cells and tissues under normal conditions, but also the preservation of all components in frozen tissue specimens. Therefore, morphofunctional information in vivo would be obtained by freeze-substituion for light or electron microscopy, and also by other analytical methods, such as freezefracture replication, X-ray microanalyses, or Raman microscopy. Considering the merits of both IVCT and cryobiopsy, their application should be expanded into other microscopic fields and also from experimental animal studies to clinical medicine.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectCryotechniqueen
dc.subjectCryobiopsyen
dc.subject.other611 - Anatomíaes
dc.titleRecent development of in vivo cryotechnique to cryobiopsy for living animalsen
dc.typeinfo:eu-repo/semantics/articlees
Appears in Collections:Vol.22, nº11 (2007)

Files in This Item:
File Description SizeFormat 
Recent development of in vivo cryotechnique to cryobiopsy for living animals.pdf4,6 MBAdobe PDFThumbnail
View/Open


Items in Digitum are protected by copyright, with all rights reserved, unless otherwise indicated.