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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | John, T. | es |
dc.contributor.author | Kohl, B. | es |
dc.contributor.author | Mobasheri, A. | - |
dc.contributor.author | Ertel, W. | - |
dc.contributor.author | Shakibaei, M. | - |
dc.date.accessioned | 2012-05-21T12:09:32Z | - |
dc.date.available | 2012-05-21T12:09:32Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0213-3911 | es |
dc.identifier.uri | http://hdl.handle.net/10201/27583 | - |
dc.description.abstract | Elevated levels of the pro-inflammatory cytokine, interleukin-18 (IL-18) have recently been demonstrated in osteoarthritic cartilage. However, the effects of IL-18 on chondrocyte signalling and matrix biosynthesis are poorly understood. Therefore, the present study was undertaken to further characterize the impact of IL-18 on human articular chondrocyte in vitro. Human articular chondrocytes were stimulated with various concentrations of recombinant human IL-18 (1, 10, 100 ng/ml) for 0, 4, 8, 12, 24, 48, 72 h in vitro. The effects of IL-18 on the cartilage-specific matrix protein collagen type II, the cytoskeletal protein vinculin, the cell matrix signal transduction receptor ß-integrin, key signalling proteins of the MAPKinase pathway (such as SHC (Sarc Homology Collagen) and activated MAPKinase [ERK-1/-2]), the pro-inflammatory enzyme cyclo-oxygenase-2 (COX-2) and the apoptosis marker activated caspase-3 were evaluated by Western blot analysis and immunofluorescence labelling. Morphological features of IL-18 stimulated chondrocytes were estimated by transmission electron microscopy. IL-18 lead to inhibition of collagen type IIdeposition, decreased ß-integrin receptor and vinculin synthesis, SHC and MAPKinase activation, increased COX-2 synthesis and activation of caspase-3 in chondrocytes in a time- and dose-dependent manner. Furthermore, chondrocytes treated with IL-18 exhibited typical morphological features of apoptosis as revealed by transmission electron microscopy. Taken together, the results of the present study underline key catabolic events mediated by IL-18 signalling in chondrocytes such as loss of cartilagespecific cartilagespecific matrix and apoptosis. Inhibition of MAPKinase signalling is hypothesized to contribute to these features. Future therapeutics targeting IL-18 signalling pathways may be beneficial in rheumatoid arthritis and osteoarthritis therapy. | es |
dc.format | application/pdf | es |
dc.format.extent | 14 | es |
dc.language | eng | es |
dc.publisher | Murcia : F. Hernández | es |
dc.relation.ispartof | Histology and histopathology | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Apoptosis | es |
dc.subject | Chondrocyte | es |
dc.subject.other | 617 - Cirugía. Ortopedia. Oftalmología | es |
dc.title | Interleukin-18 induces apoptosis in human articular chondrocytes | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.22, nº 5 (2007) |
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Interleukin18 induces apoptosis in human articular chondrocytes.pdf | 5,43 MB | Adobe PDF | Visualizar/Abrir |
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