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Title: Immunohistochemical analyses on serum proteins in nephrons of protein-overload mice by in vivo cryotechnique
Issue Date: 2007
Publisher: Murcia : F. Hernández
ISSN: 0213-3911
Related subjects: 616.4 - Patología del sistema linfático, órganos hematopéyicos, endocrinos
Keywords: Immunohistochemistry
Abstract: Immunohistochemical analyses on local distributions of serum proteins in living mouse kidneys are usually difficult to examine with conventional preparation methods. By using our “in vivo cryotechnique” combined with freeze-substitution, we have checked immunolocalizations of the serum proteins in nephrons of bovine serum albumin (BSA)-overload mice, and compared them with those obtained by the conventional preparation methods. In two days of daily BSA-injected mice, the immunolocalization of BSA could be observed in Bowman’s space and urinary tubules with their overt proteinuria, where another endogenous mouse albumin was similarly immunolocalized. The leakage of BSA and mouse albumin in Bowman’s space and their reabsorption into proximal tubules were detected in 55% of nephrons, where no leakage of immunoglobulin G1 (IgG1) was detected. However, the leakage of IgG1, in addition to BSA and mouse albumin, was detected in the other nephrons. By carefully examining immunolocalizations of BSA and IgG1, they were obviously different from those obtained by the conventional preparation methods without normal blood circulation into the kidneys. The immunolocalizations of both BSA and mouse serum proteins could be directly analyzed with the “in vivo cryotechnique”, suggesting that functional damage to glomerular filtration barriers are different at early stages of the BSA-overload mouse model, depending on each nephron of living mice.
Primary author: Zhou, D.S.
Ohno, N.
Terada, N.
Li, Z.
Morita, H.
Inui, K.
Yoshimura, A.
Ohno, S.
Published in: Histology and histopathology
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 9
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Vol.22, nº 2 (2007)

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