Please use this identifier to cite or link to this item: http://hdl.handle.net/10201/22627

Title: Application of in vivo cryotechnique to the examination of cells and tissues in living animal organs
Issue Date: 2006
Publisher: Murcia : F. Hernández
ISSN: 0213-3911
Related subjects: CDU::6 - Ciencias aplicadas::61 - Medicina::611 - Anatomía
Keywords: In vivo cryotechnique
Immunolocalization
Abstract: When all biological materials in cells and tissues of living animal organs are quickly and promptly frozen, immunolocalization of their components and structural features in situ is necessary to understand their in vivo functioning states. However, these direct morphological analyses were difficult to achieve by conventional chemical fixation methods during the last century. A new cryofixation method, named the “in vivo cryotechnique”, in which the normal blood circulation in living animals is always retained at the moment of freezing, has become a powerful tool to visualize the real native morphology of cells and tissues with functional meaning. The “in vivo cryotechnique” can usually be combined with a wide range of subsequent preparation techniques, and can thereby enable us to perform various direct analyses on biological samples, reflecting the physiological functions of living animal organs
Primary author: Terada, N.
Ohno, N.
Li, Z.
Fuji, Y.
Baba, T.
Ohno, S.
Published in: Histology and histopathology
URI: http://hdl.handle.net/10201/22627
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 8
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Vol.21, nº 3 (2006)



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