Please use this identifier to cite or link to this item: http://hdl.handle.net/10201/21511

Title: Immunohistochemical identification of intracytoplasmic lumens by cytokeratin typing may differentiate renal oncocytomas from chromophobe renal cell carcinomas
Issue Date: 2004
Publisher: Murcia : F. Hernández
ISSN: 0213-3911
Related subjects: CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología::616.6 - Patología del sistema genitourinario
Keywords: Intracytoplasmic lumen
Renal oncocytomas
Abstract: Renal oncocytomas and chromophobe renal cell carcinomas (RCCs) share a common phenotype and both originate from the intercalated cells of the collecting duct. This makes it very difficult to differentiate between the two tumors immunohistochemically. Therefore, we studied the results of immunohistochemistry focusing on certain characteristic structures that are occasionally present in renal oncocytomas. We carried out Hale’s colloidal iron staining and immunohistochemistry for various cytokeratins (cytokeratins 7, 8, 10, 10/13, 14, 18, 19 and 20, and AE1/AE3) in four oncocytomas and six chromophobe RCCs. In addition, one renal oncocytoma and one chromophobe RCC were studied using electron microscopy. Two renal oncocytomas and one chromophobe RCC were completely unstained by colloidal iron. There was no evident difference between the immunohistochemical characteristics of oncocytomas and those of chromophobe RCCs. However, in all four renal oncocytomas we identified intracytoplasmic ring-like positive reactions for some cytokeratins (at least 3 antigens of cytokeratins 7, 8 and 19, and AE1/AE3), which corresponded ultrastructurally to the intracytoplasmic lumens (ICLs). In contrast, no such structures were found in any of the chromophobe RCCs using the antibodies employed. Therefore, immunohistochemical identification of ICLs by cytokeratin typing may be useful for differentiating between renal oncocytomas and chromophobe RCCs and be more sensitive in this respect than colloidal iron staining.
Primary author: Kuroda, Naoto
Toi, M.
Yamamoto, M.
Miyazaki, E.
Hayashi, Yoshihiro
Hiroi, Makoto
Shuin, T.
Enzan, H.
Published in: Histology and histopathology
URI: http://hdl.handle.net/10201/21511
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 6
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Vol.19, nº 1 (2004)

Files in This Item:
File Description SizeFormat 
Immunohistochemical identification of intracytoplasmic.pdf3,08 MBAdobe PDFView/Open


Items in Digitum are protected by copyright, with all rights reserved, unless otherwise indicated.