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dc.contributor.authorThiele, J.es
dc.contributor.authorWickenhauser, C.es
dc.contributor.authorKvasnicka, H.M.-
dc.contributor.authorVarus, E.-
dc.contributor.authorBeelen, D.W.-
dc.contributor.authorSchaefer, U.W.-
dc.date.accessioned2011-06-08T09:02:36Z-
dc.date.available2011-06-08T09:02:36Z-
dc.date.issued2003-
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/21411-
dc.description.abstractScant knowledge is available about the dynamics of lineage-specific mixed chimerism (Ch) following bone marrow transplantation (BMT). This review is focused on findings derived from bone marrow (BM) biopsies in patients with chronic myeloid leukemia (CML) including a sex-mismatched host/donor constellation. Appropriate techniques involved immunophenotyping by monoclonal antibodies to identify the various cell lineages, dual color fluorescence in situ hybridization (FISH) with x- and y-chromosomespecific DNA-probes and a proper detection system for a simultaneous labeling of the bcr/abl locus. A significant degree of Ch with more than 20% host CD34+ progenitors was found in the early and late (up to 200 days after BMT) posttransplant period. However, only 10% of these cells harbored the bcr/abl translocation gene. This result fits well with corresponding molecularbiological findings of so-called minimal residual disease. Conversion of Ch evolved during leukemic relapse with 90% host progenitors of which 50% revealed the bcr/abl locus. A Ch of nucleated erythroid percursors (5%) and CD68+ macrophages (8%) was expressed to a significantly lower degree. The slightly increased frequency found in CD61+ megakaryocytes (16%) was probably due to the polyploid state of these cells. Similar to the CD34+ progenitor cells abrupt changes from donor to host type was associated with an insidious transformation into recurrent leukemia. The CD34+ endothelial cells showed a minor degree of Ch, because donor-derived elements ranged from 18% to 25%. Leukemic relapse was characterized by an almost complete conversion of the endothelial cells to a host type. These findings point towards a CD34+ progenitor cell origin of the (leukemic) endothelial cell layer and suggests that their dysfunction may contribute to an expansion of the neoplastic clone.es
dc.formatapplication/pdfes
dc.format.extent18es
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectMixed chimerismes
dc.subjectErythroid precursorses
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleDynamics of lineage-restricted mixed chimerism following sex-mismatched allogeneic bone marrow transplantationes
dc.typeinfo:eu-repo/semantics/articlees
Aparece en las colecciones:Vol.18, nº 2 (2003)

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