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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Sasso-Cerri, E. | es |
dc.contributor.author | Miraglia, S.M. | es |
dc.date.accessioned | 2011-05-11T10:58:02Z | - |
dc.date.available | 2011-05-11T10:58:02Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0213-3911 | es |
dc.identifier.uri | http://hdl.handle.net/10201/20778 | - |
dc.description.abstract | Cimetidine has caused dysfunction in the male reproductive system. In the rat testis, intratubu l a r alterations and loss of peritubular tissue due to p e r i t u bular myoid cell death by apoptosis have been recently shown. Thus, the aim of this study is to evaluate which cells of the seminiferous epithelium have been affected and/or died by apoptosis after the treatment with cimetidine. For this purpose, an experimental group containing five male albino Wistar rats received intraperitoneal injections of cimetidine (50 mg/kg body weight) during 52 days. The testes were f i xed with 4% bu ff e r e d f o r m a l d e hyde and were embedded in paraffin. Fo r detection of DNA breaks (apoptosis) in the cells of the seminiferous epithelium, the testicular sections were treated by the TUNEL method (Apop-Tag Plus Peroxidase Kit). In the tubules affected by cimetidine, altered p e r i t u bular tissue, including the presence of TUNEL labeling in the myoid peritubular cells, were usually found. In these tubules, the seminiferous epithelium exhibited low density of germ cells and TUNEL-positive labeling in the germ cells of the basal compartment. The concomitant staining in both germ cells of the basal compartment and late spermatids suggest a sensitivity of these cells in the damaged tubules. Besides germ cells, T U N E L - p o s i t ive Sertoli cells were also found in the injured seminiferous tubules. Thus, a relationship between dying germ cells and Sertoli cell damage and/or death must be considered in tubules where peritubu l a r tissue has been affected by toxicants. | es |
dc.format | application/pdf | es |
dc.format.extent | 7 | es |
dc.language | eng | es |
dc.publisher | Murcia : F. Hernández | es |
dc.relation.ispartof | Histology and histopathology | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Germ cells | es |
dc.subject | Sertoli cell | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina | es |
dc.title | In situ demonstration of both TUNEL-labeled germ cell and Sertoli cell in the cimetidine-treated rats | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.17, nº 2 (2002) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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In situ demonstration of both TUNELlabeled.pdf | 1,9 MB | Adobe PDF | Visualizar/Abrir |
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