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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Dunk, C. | es |
dc.contributor.author | Ahmed, A. | es |
dc.date.accessioned | 2011-05-11T10:56:04Z | - |
dc.date.available | 2011-05-11T10:56:04Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0213-3911 | es |
dc.identifier.uri | http://hdl.handle.net/10201/20664 | - |
dc.description.abstract | Placental villous development requires the co-ordinated action of angiogenic factors on both endothelial and trophoblast cells. Like vascular endothelial growth factor (VEGF), VEGF-C increases vascular permeability, stimulates endothelial cell proliferation and migration. In the present study, we investigated the expression of VEGF-C and its receptors VEGFR-3 and VEGFR-2 in normal and intrauterine growth-restricted (IUGR) placenta. Immunolocalisation studies showed that like VEGF and VEGFR-1, VEGF-C, VEGFR-3 and VEGFR-2 co-localised to the syncytiotrophoblast, to cells in the maternal decidua, as well as to the endothelium of the large placental blood vessels. Western blot analysis demonstrated a significant decrease in placental VEGF-C and VEGFR-3 protein expression in severe IUGR as compared to gestationallymatched third trimester pregnancies. Conditioned medium from VEGF-C producing pancreatic carcinoma (Suit-2) and endometrial epithelial (Hec-1B) cell lines caused an increased association of the phosphorylated extracellular signal regulated kinase (ERK) in VEGFR-3 immunoprecipitates from spontaneously transformed first trimester trophoblast cells. VEGF121 caused dosedependant phosphorylation of VEGFR-2 in trophoblast cells as well as stimulating DNA svnthesis. In addition. premixing VEGFl with Yheparin sÚlphate proteoglycan wtentiated trooho8ast oroliferation and the association Lf p h ~ s p hw~ith- t i~e V~E~GF R-2 receptor. VEGF165- mediated DNA synthesis was inhibited by anti-VEGFR- 2 neutralising antibody. The results demonstrate functional VEGFR-2 and VEGFR-3 receptors on trophoblast and suggest that the decreased expression of VEGF-C and VEGFR-3 may contribute to the abnormai villous development observed in IUGR placenta. | es |
dc.format | application/pdf | es |
dc.format.extent | 17 | es |
dc.language | eng | es |
dc.publisher | Murcia : F. Hernández | es |
dc.relation.ispartof | Histology and histopathology | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Placenta | es |
dc.subject | Endothelial cells | es |
dc.subject.other | CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología | es |
dc.title | Expression of VEGF-C and activation of its receptors VEGFR-2 and VEGFR-3 in trophoblast | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.16, nº 2 (2001) |
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Expression of VEGFC and activation of its.pdf | 10,99 MB | Adobe PDF | Visualizar/Abrir |
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