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dc.contributor.authorSpangeus, A.es
dc.contributor.authorForsgren, Sturees
dc.contributor.authorEl-Salhy, M.-
dc.date.accessioned2011-05-11T10:55:53Z-
dc.date.available2011-05-11T10:55:53Z-
dc.date.issued2001-
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/20655-
dc.description.abstractChanges in the numbers of serotonin- and substance P-immunoreactive (IR) cells occur in severa1 animal models of diabetes. It is not known, however, whether these changes are a result of actual ceii loss or are caused by modified gene expression in ceíls showing co-localization of serotonin and substance P. The pattern of mono- and co-expression of serotonin, as well as of substance P, was therefore investigated in gastrointestinal endocrine cells from animal models of human type 1 and type 2 diabetes, namely non-obese diabetic (NOD) and obese diabetic (oblob) mice. Immunocytochemical staining by the avidin-biotin complex method was performed for computerized image analysis of each cell type, and by immunofluorescence double staining to study co-localization. Tissues from antrum, proximal duodenum and dista1 colon were investigated. Co-localization of serotonin- and substance P-IR was found in al1 investigated parts of the gut. In antrum, substance P immunoreactivity was found exclusively in serotonin-IR cells. In both NOD and oblob mice there was a reduced number of substance PIR cells, but an unchanged serotonin-IR cell count, which thus tallies with a shut-off of substance P expression in antral enterochromaffin cells. In duodenum, both diabetes models showed a decreased number of serotonin-IR cells. Furthermore there was a decreased number of substance P-IR cells in the type 2 model. The proportion of serotonin-IR cells showing substance P-immuno-reactivity was decreased in both diabetic models, thus indicating a shut-off of substance P-gene expression. However, this does not fully explain the changes in duodenum, but the diabetic state probably affects the number of mono-expressed cells as well. In colon, no change was found in diabetic mice regarding co-localization of substance P and serotonin. However, pre-diabetic NOD mice showed a decreased proportion of substance P in serotonin-IR cells, which might be explained by the increased number of serotonin-IR cells, combined with an unchanged number of substance P-IR cells. In conclusion, diabetic animal models of both type 1 and type 2 appear to have a combination of decreased expression of substance P in serotonin-IR cells of both antrum and duodenum, as well as a change in the number of mono-expressed cells. The pattern in colon, on the other hand, seems to be unaffected.es
dc.formatapplication/pdfes
dc.format.extent6es
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectDiabeteses
dc.subjectSubstance Pes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleEffect of diabetic state on co-localization of substance P and serotonin in the gut in animal modelses
dc.typeinfo:eu-repo/semantics/articlees
Appears in Collections:Vol.16, nº 2 (2001)

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