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http://hdl.handle.net/10201/20633
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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Yamashita, S. | es |
dc.contributor.author | Takayanagi, A. | es |
dc.contributor.author | Shimizu, N. | - |
dc.date.accessioned | 2011-05-11T10:55:30Z | - |
dc.date.available | 2011-05-11T10:55:30Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0213-3911 | es |
dc.identifier.uri | http://hdl.handle.net/10201/20633 | - |
dc.description.abstract | Quantitative and cell-type-specific expression of c-fos and c-jun genes after 17B-estradiol (E2) stimulation, was investigated in the uteri of neonatally diethylstilbestrol (DES)-exposed and ovariectomized adult mice (neoDES-mice), employing Northern blot analysis, immunohistochemistry and in situ hybridization. The c-fos mRNA level before E2 injection (at baseline) was about 2.2-fold higher in neoDES-mice than in vehicle-treated control mice. In controls, E2 treatment transiently increased c-fos mRNA levels, showing a peak value (15.8-fold relative to the baseline) after 2 hours. In neoDES-mice, c-fos mRNA level reached a peak showing a 2.1-fold increase compared with its baseline value 1 hour after E2 injection. Immunohistochemistry and in situ hybridization revealed that c-fos protein (Fos) and mRNA are induced in the epithelium and vascular endothelium in both groups. Most uterine epithelia of neoDES-mice revealed low sensitivity to the c-fos expression after E2 administration compared with those of vehicle-treated controls, whereas few epithelia showed high c-fos mRNA expression even at baseline. The c-jun mRNA concentration in the neoDES-mice uteri at baseline was 70% of that in vehicle-treated controls. At 1 hour after E2 injection, c-jun mRNA levels increased 1.8-fold in controls and 1.3-fold in the neoDES-mice relative to each baseline value. There were no significant differences in the distribution pattern of cjun protein (Jun) and mRNA in the uteri of either groups; E2 stimulated c-jun mRNA expression in the stromal and myometrial cells but suppressed it in the epithelial cells, whereas intensity of c-jun immunostaining increased in the three cell types. The permanent changes in the expression of estrogen-regulated protooncogenes, c-fos and c-jun genes, by neonatal DES exposure may be responsible for the wide range of abnormalities in the genital tract of mature animals. | es |
dc.format | application/pdf | es |
dc.format.extent | 10 | es |
dc.language | eng | es |
dc.publisher | Murcia : F. Hernández | es |
dc.relation.ispartof | Histology and histopathology | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Diethylstilbestrol | es |
dc.subject | Uterus | es |
dc.subject.other | CDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísica | es |
dc.title | Effects of neonatal diethylstilbestrol exposure on c-fos and c-jun protooncogene expression in the mouse uterus | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.16, nº 1 (2001) |
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Fichero | Descripción | Tamaño | Formato | |
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Effects of neonatal diethylstilbestrol exposure on cfos and cjun protooncogene expression in the mouse uterus.pdf | 7,37 MB | Adobe PDF | ![]() Visualizar/Abrir |
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