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Title: Development of fast and simple methods for porcine haptoglobin and ceruloplasmin purification
Other Titles: Desarrollo de métodos rápidos y sencillos para la purificación de haptoglobina y ceruloplasmina porcina
Issue Date: 2010
Publisher: Murcia, Universidad de Murcia, Servicio de Publicaciones
ISSN: 1989-1784 (electrónico)
Related subjects: CDU::6 - Ciencias aplicadas::63 - Agricultura. Silvicultura. Zootecnia. Caza. Pesca::636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticos
Keywords: Porcine
Abstract: Two fast and simple methods for porcine haptoglobin (Hp) and ceruloplasmin (Cp) purification are described in this paper. Hp was purified by ammonium sulphate fractionation and a FPLC Superdex-200 gel chromatography. The protein obtained showed two bands with a Mr (molecular mass) of about 44 kDa and 12.9 kDa corresponding to heavy (ß) and light (a) chains of Hp respectively, on sodium dodecyl sulphate-polyacrilamide gel electrophoresis, under reducing conditions. Ceruloplasmin (Cp) was isolated by one step of chromatography on amino-ethyl-derivatized Sepharose followed by gel filtration on a Superdex-200 column. The Mr of the protein, as estimated by SDS-PAGE was approximately 150 kDa. In conclusion, two new protocols have been developed for porcine Hp and Cp purification, being less time-consuming and technically demanding than those previously reported. This paper could represent an interesting guideline and be of help to obtain pure protein to use as specie-specific standard material and to produce specific antibodies.
Primary author: Fuentes, P.
Gutiérrez Andrés, M.
Soler, L.
Cerón Madrigal, José Joaquín
Martínez Subiela, Silvia
Faculty / Departments / Services: Facultad de Veterinaria
Published in: Anales de veterinaria de Murcia,
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 12
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Vol. 26 (2010)

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