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Título: | Freeze-fracture cytochemistry,a new fracture-labeling method for topological analysis of biomembrane molecules |
Fecha de publicación: | 2000 |
Editorial: | Murcia : F. Hernández |
ISSN: | 0213-3911 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina |
Palabras clave: | Immunocytochemistry Enzyme cytochemistry |
Resumen: | Freeze-fracture cytochemistry allows visualization of cellular and molecular characteristics of biomembranes in situ. In this review, we discuss freezefracture cytochemistry with special reference to a new cytochemical labeling of replicas, the detergentdigestion fracture-labeling technique. In this procedure, unfixed cells are rapidly-frozen, freeze-fractured, and physically stabilized by evaporated platinum/carbon. The frozen cells are then removed from the freezefracture apparatus to thaw and are subsequently treated with detergents. After detergent-digestion, replicas are labeled with cytochemical markers. We demonstrate that the technique is a versatile tool for direct analysis of the macromolecular architecture of biomembranes and allows identification of particular intracellular membrane organelles. In addition, we demonstrate the application of ultrasmall gold to freeze-fracture immunocytochemistry. Freeze-fracture cytochemistry is a valuable technique for investigating topology and dynamics of membrane molecules. |
Autor/es principal/es: | Takizawa, T. Robinson, J.M. |
Forma parte de: | Histology and histopathology |
URI: | http://hdl.handle.net/10201/19302 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 8 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol.15, nº 2 (2000) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Freezefracture cytochemistrya new fracturelabeling method for topological analysis of biomembrane molecules.pdf | 455,05 kB | Adobe PDF | Visualizar/Abrir |
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