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Título: | The molecular determinants of the efficiency of green fluorescent protein mutants |
Fecha de publicación: | 2000 |
Editorial: | Murcia : F. Hernández |
ISSN: | 0213-3911 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología |
Palabras clave: | GFP FRET |
Resumen: | The Green Fluorescent Protein (GFP) is a spontaneously fluorescent polypeptide of 27 kD from the jellyfish Aequorea victoria that absorbs UV-blue light and emits in the green region of the spectrum. GFP has been succesfully expressed both in bacteria and in eukaryotic cells and is widely used to monitor the localization of tagged proteins in living cells. Since wtGFP performs inefficiently in different cellular contexts, efforts have been devoted to the improvement of GFP expression levels andtor fluorescence. We will here review the basic characteristics of wt and mutated GFP, in particular their protein expression vs fluorescent properties. Emphasis will be given to unexpected consequences of mutations of the GFP gene, i.e. on transcription and translation rates and on protein folding in different cell types, and to how these critically reflect on the use of GFP in different cellular environments. |
Autor/es principal/es: | Sacchetti, A. Ciccocioppo, R. Alberti, S. |
Forma parte de: | Histology and histopathology |
URI: | http://hdl.handle.net/10201/19272 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 7 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol.15, nº 1 (2000) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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The molecular determinants of the efficiency of green fluorescent protein mutants.pdf | 4,1 MB | Adobe PDF | Visualizar/Abrir |
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