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Title: Does diabetic state affect CO-localization of peptide W and enteroglucagon in colonic endocrine cells?
Issue Date: 2000
Publisher: Murcia : F. Hernández
ISSN: 0213-3911
Related subjects: CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología
Keywords: Diabetes
Abstract: Background: Changes in the numbers of PYY- and enteroglucagon-immunoreactive cells in colon of animal models of human diabetes have been reported. As these peptides CO-localize in the same cells it is possible that the observed changes are a result of changes in CO-localization. Methods: Animal models of human type 1 and type 2 diabetes, namely the non-obese diabetic (NOD) mouse and the obese (oblob) mouse, were studied. As controls for the NOD mice, BALBIcJ mice were used and for oblob mice, homozygous lean (+l+) mice were used. Tissue samples from colon were double-immunostained for PYY and enteroglucagon according to the indirect immunofluorescence method. Results: CO-localization of enteroglucagon and PYY was found in colonic endocrine cells in all groups investigated. Compared with controls, pre-diabetic NOD mice showed a decreased proportion of enteroglucagon1PYY CO-localization. There was no difference in diabetic NOD mice or diabetic oblob mice when compared with controls. Conclusions: Whereas the number of cells containing solely enteroglucagon and solely PYY increases in pre-diabetic NOD mice, production of enteroglucagon in PYY-immunoreactive cells decreases. Although the numbers of PYY and enteroglucagon cells have been reported to be changed in both diabetic NOD mice and in obese mice, the balance between CO-expressing and mono-expressing cells seems to be preserved.
Primary author: Spangeus, A.
Forsgren, Sture
El-Salhy, M.
Published in: Histology and histopathology
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 5
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Vol.15, nº 1 (2000)

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