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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Trujillo, E. | es |
dc.contributor.author | Alvarez de la Rosa, D. | es |
dc.contributor.author | Mobasheri, A. | - |
dc.contributor.author | Ávila, J. | - |
dc.contributor.author | Gonzalez, T. | - |
dc.contributor.author | Martín Vasallo, P. | - |
dc.date.accessioned | 2011-02-22T11:09:58Z | - |
dc.date.available | 2011-02-22T11:09:58Z | - |
dc.date.issued | 1999 | - |
dc.identifier.issn | 0213-3911 | es |
dc.identifier.uri | http://hdl.handle.net/10201/19239 | - |
dc.description.abstract | The chondrocyte is the cell responsible for the maintenance of the articular cartilage matrix. The negative charges of proteoglycans of the matrix draw cations, principally Na+, into the matrix to balance the negative charge distribution. The Na+,Kf -ATPase is the plasma membrane enzyme that maintains the intracellular Na+ and K+ concentrations. The enzyme is composed of an a and a l3 subunit, so far, 4 a and 3 B isoforms have been identified in mammals. Chondrocytes are sensitive to their ionic and osmotic environment and are capable of adaptive responses to ionic environmental perturbations particularly changes to extracellular [Na+]. In this article we show that human fetal and adult chondrocytes express three a ( a l , a 2 and the neural form of a3) and the three l3 isoforms (131, l32 and 83) of the Na+,K+-ATPase. The presence of multiple Na+,K+-ATPase isoforms in the plasma membrane of chondrocytes suggests a variety of kinetic properties that reflects a cartilage specific and very fine specialization in order to maintain the Na+/K+ gradients. Changes in the ionic and osmotic environment of chondrocytes occur in osteoarthritis and rheumatoid arthritis as result of tissue hydration and proteoglycan loss leading to a fall in tissue Na+ and K+ content. Although the expression levels and cellular distribution of the proteins tested do not vary, we detect changes in p-nitrophenylphosphatase activity "in situ" between control and pathological samples. This change in the sodium pump enzymatic activity suggests that the chondrocyte responds to these cationic environmental changes with a variation of the active isozyme types present in the plasma membrane. | es |
dc.format | application/pdf | es |
dc.format.extent | 12 | es |
dc.language | eng | es |
dc.publisher | Murcia : F. Hernández | es |
dc.relation.ispartof | Histology and histopathology | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Osteoarthritis | es |
dc.subject | Rheumatoid arthritis | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina | es |
dc.title | Sodium transport systems in human chondrocytes Morphological and functional expression of the Na+,K+-ATPase a and D subunit isoforms in healthy and arthritic chondrocytes | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.14, nº 4 (1999) |
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