Por favor, use este identificador para citar o enlazar este ítem:
http://hdl.handle.net/10201/19161
Twittear
Título: | lmmunocytochemical distribution of cytochrome P4501A CYPlA in developing gilthead seabream, Sparus aurata |
Fecha de publicación: | 1999 |
Editorial: | Murcia : F. Hernández |
ISSN: | 0213-3911 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina |
Palabras clave: | Immunohistochemistry Tissues |
Resumen: | CYPlA is a major inducible enzyme in the metabolism of xenobiotic substrates. In this paper we investigate by means of immunohistochemistry, the tissue distribution of constitutive cytochrome P4501A (CYPlA) during the period of endogenous nutrition (from hatching until day 4) in developing gilthead seabream, Sparus aurata larvae. For this purpose, a polyclonal antiserum (BN- 1, Biosense Laboratories) directed against conserved piscine CYPlA sequences was used on paraffin-embedded sections from seabream larvae. From hatching onward, CYPlA immunoreactivity was observed in the following tissues and cells: syncytial, oil-globule envelopes and matrix of the yolk-sac, kidney (epithelia of renal tubules), cardiac muscle cells, skin epidermal cells, troncal musculature, enterocytes of different intestinal regions, goblet cells of the bucco-pharyngeal region, gill epithelia1 cells and the endothelia of the vascular system of various tissues (especially from liver and brain). Moreover, eye (retina), olfactory epithelium and some positive nerve fibers located in the proximity of the olfactory bulbs and running ventrally toward the posterior brain were strongly CYPlA immunoreactive. In general, the intensity of immunostaining increased with larval development. |
Autor/es principal/es: | Sarasquete, C. Muñoz-Cueto, J.A. Ortiz Delgado, J.B. Rodriguez-Gomez, F.J. Dinis, M.T. Segner, H. |
Forma parte de: | Histology and histopathology |
URI: | http://hdl.handle.net/10201/19161 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 9 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol.14, nº 2 (1999) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
lmmunocytochemical distribution of cytochrome P4501A CYPlA in developing gilthead seabream Sparus aurata.pdf | 5,4 MB | Adobe PDF | Visualizar/Abrir |
Los ítems de Digitum están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.