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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Sarasquete, C. | es |
dc.contributor.author | Gonzalez de Canales, M.L. | es |
dc.contributor.author | Arellano, J.M. | - |
dc.contributor.author | Pérez-Prieto, S. | - |
dc.contributor.author | García-Rosado, E. | - |
dc.contributor.author | Borrego, J.J. | - |
dc.date.accessioned | 2011-02-22T10:58:02Z | - |
dc.date.available | 2011-02-22T10:58:02Z | - |
dc.date.issued | 1998 | - |
dc.identifier.issn | 0213-3911 | es |
dc.identifier.uri | http://hdl.handle.net/10201/19031 | - |
dc.description.abstract | A battery of horseradish peroxidasecorijugated lectiris (Con A, WGA arid DBA). as well as coriventional histochemical techniques (PAS. saponification, Alcian Blue pH 0.1 , 1. 2.5. chlorhydric hydrolisis. sialidasc, Bromophenol blue, Tioglycollate reduction and Ferric-ferricyanide-FeIlI) were used to study the coritent and distribution of carbohydrates, proteins and glycoconjugatc sugar residues on the skin and on the lymphocystis-infected cells of gilthead seabrcain, Spcrrus uurntcr. Variable ari-iounts of glycoproteins containing sialic acid, N-acetyl-Dglucosainine, N-acetyl-D-galactosarnine; mannose andlor glucose residues were observed in the cuticle and mucous cells of the c o r ~ o r a ls kin. tails and f ins . ~ ~ Germinative and epithelial cells of the epidermis contained glycogen. proteins. carboxylated groups, as well as glycoproteins with mannose andlor glucose and N-acetyl-U-galactosamine residues. Hyaline capsule of the mature lyniphocystis-infected cells was strongly stained with PAS, Alcian Blue (pH 0.5 and 2.5) and weakly positive with Alcian Blue (pH 1 ) . Con A reacted with the granular cytoplasm, specially around hyaline capsule. and with the basophilic intracytoplasinic inclusions developed in mature lymphocystis-infected cells of Spcrrrrs crlirntu skin. These sugar residues (niannose andlor glucose), as well as N-acetyl-Drrlucosamine andlor sialic acid and N-acetvl-D- L galactosamine were not detected in the hyaline capsule of the lyr.iphocysti5 disease. | es |
dc.format | application/pdf | es |
dc.format.extent | 9 | es |
dc.language | eng | es |
dc.publisher | Murcia : F. Hernández | es |
dc.relation.ispartof | Histology and histopathology | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Skin | es |
dc.subject | Glycoproteins | es |
dc.subject.other | CDU::5 - Ciencias puras y naturales::57 - Biología | es |
dc.title | Histochemical study of lymphocystis disease in skin of gilthead seabream, Sparus aurata L. | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.13, nº 1 (1998) |
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Histochemical study of lymphocystis disease in skin of gilthead seabream Sparus aurata L..pdf | 811,65 kB | Adobe PDF | Visualizar/Abrir |
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