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http://hdl.handle.net/10201/18796


Title: | lmmunogold analysis of antioxidant enzymes in common renal cancers |
Issue Date: | 1996 |
Publisher: | Murcia : F. Hernández |
ISSN: | 0213-3911 |
Related subjects: | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología |
Keywords: | Immunogold techniques Kidney cancer |
Abstract: | Immunogold studies of normal human kidney and common human kidney cancers were performed using polyclonal antibodies to antioxidant enzymes, including antibodies to copper, zinc and manganese superoxide dismutases, catalase, glutathione peroxidase, and glutathione S-transferases and their subunits. Normal tissue adjacent to human renal tumors had the same antioxidant enzyme immunoreactive protein profiles as normal human kidney, thus establishing that the presence of tumor does not alter the levels of antioxidant enzyme immunoreactive proteins in adjacent kidney tissue. Levels of immunoreactive protein for antioxidant enzymes were determined in four common types of malignant renal cancer. In general, tumors had low levels of antioxidant enzymes; however, certain histologic types of renal tumors had high levels of immunoreactive protein for glutathione S-transferase subunits, which could affect their susceptibility to chemotherapy. Studies of transitional carcinoma of the renal pelvis were especially informative since it was possible to compare levels of antioxidant enzyme immunoreactive protein with adjacent normal transitional epithelium; the majority of antibodies resulted in lower levels of immunoreactive protein in transitional cell carcinoma than in adjacent normal transitional epithelium. Our results are discussed in relation to the response of renal tumors to therapy. |
Primary author: | Oberley, T.D. Sempf, J.M. Oberley, L.W. |
Published in: | Histology and histopathology |
URI: | http://hdl.handle.net/10201/18796 |
Document type: | info:eu-repo/semantics/article |
Number of pages / Extensions: | 8 |
Rights: | info:eu-repo/semantics/openAccess |
Appears in Collections: | Vol.11, nº 1 (1996) |
Files in This Item:
File | Description | Size | Format | |
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lmmunogold analysis of antioxidant enzymes in common renal cancers.pdf | 5,25 MB | Adobe PDF | ![]() View/Open |
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