Modifications of the dermis during scale regeneration in the lizard tail

Abstract

During scale morphogenesis in the regenerating tail of lizards (Anolis and Lampropholis) the structure of the dermis undergoes changes in relation to the ingrowth of epidermal papillae to form the new scales. Cell proliferation in the dermis, as revealed by the uptake of 3~-thymidinei,s high in the prescaling region of the regenerating tail but lower than the proliferation in the epidermis. Under the epidermis of the scaling region dermal cell proliferation rapidly drops down under the distal (apical) and proximal (caudal) sides of the infoldin epidermal papillae. Dermal fibroblasts take up g ~ - p r o l i n ein high amounts, especially in the forming deep dermal layer, where many collagen fibrils are laid down forming dense connective. Electron microscopic study revealed that ((anchoring filaments~li nk the basement membrane of the epidermis with the deep dermis, in particular in the sinking hnge region. As a result of the higher proliferation of the epidermis with respect to the dermis (heterochrony) and the presence of dermo-epithelial eanchoring filarnents», the superficial laminar epidermis sinks into the dermis to produce new scales. The epidermal downpushing is evidenced by a characteristic distortion of the dermal fibrils under the distal and the proximal sides, and in the hinge region of the forming scales