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Título: | Seeding of expanded polytetrafluoroethylene (ePTFE) vascular grafts. A morphological study of porcine endothelial and fibroblast cells |
Fecha de publicación: | 1992 |
Editorial: | Murcia : F. Hernández |
ISSN: | 0213-3911 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina |
Palabras clave: | Endothelial cells Cell seeding |
Resumen: | The need to improve clinical results with small and medium calibre grafts has led to extensive research on cell seeding of prosthetic materials. Numerous problems remain regarding identification, seeding, adhesion and survival of the cells attached. We have studied the behaviour of seedings of endothelial and fibroblast cells on ePTFE grafts. Scanning electron microscopy allows us to observe the morphological characteristics and their interaction with the biopolymers. It has been possible to differentiate both cellular types by their characteristics and interactions with the ePTFE. At the same time, from this ~ i vni t ro~st udy it can be concluded that the time needed to obtain a stable and confluent monolayer on ePTFE pretreated with fibronectin is between 18 hours to 4 days for endothelial cells, and 24 hours for fibroblasts. These would be the optimal time periods for ain vivo. grafting of the seeded prostheses. |
Autor/es principal/es: | Buján, J. Bellón, J.M. Navlet, J.G. Honduvilla, N. Hernando, A. Turegano, F. |
Forma parte de: | Histology and histopathology |
URI: | http://hdl.handle.net/10201/18351 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 8 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol. 7, nº 4 (1992) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Seeding of expanded polytetrafluoroethylene ePTFE vascular grafts. A morphological study of porcine endothelial and fibroblast cells.pdf | 4,94 MB | Adobe PDF | Visualizar/Abrir |
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