Morphology and functional responses of isolated inner adrenocortical cells of rats infuse with interleukin-B

Abstract

The effects of the prolon Ye d infusion with interleukin-1B (IL-10) (20 pM.kg- .min- ') on the function and morphology of the isolated inner cells of the rat adrenal cortex were investigated. After 3 and 5 days of IL-1B infusion, the level of circulating ACTH was below the control level, while the plasma concentration of corticosterone was strikingly elevated. After 5 days of infusion, isolated inner adrenocortical cells showed an enhanced basal and ACTH-stimulated corticosterone secretion, and showed a conspicuous hypertrophy. The acute exposure to IL-1B 10" M did not affect the secretory activity of dispersed cell from either control or IL-lB-infused rats. These findings indicate that the prolonged exposure to high levels of circulating IL-lB, like those occurring during chronic inflammatory diseases, is able to enhance the growth and steroidogenic (glucocorticoid) capacity of the rat inner adrenocortical zones. Moreover, they suggest that the mechanism underlying this adrenocorticotrophic effect of IL-1B does not involve either a stimulation of the hypophyseal ACTH release or a direct stimulatory effect of monokine on adrenocortical cells. It is suggested that IL-1B may activate an intra-adrenal paracrine regulatory mechanism.