Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10201/18220

Título: Morphology, differentiation and matrix production of liver cells in organoid cultures high density cultures of fetal rat livers
Fecha de publicación: 1991
Editorial: Murcia : F. Hernández
ISSN: 0213-3911
Materias relacionadas: CDU::6 - Ciencias aplicadas::61 - Medicina
Palabras clave: Fetal rat
Liver
Resumen: Tlie aim of this study \\as to dcn~ons t ratetl ie morpl~ologya nd matrix synthesis of ernl~ryonicr at liver cc.11\ (clay IS of gestation) in organoid cultures (high densit!, cultures) with electron microscopic and imm~~nomorphologictaelc hniclues. For this purpose tlie cells of embryonic rat livers were isolated enzyniaticall! and g r o ~i1~1 ;iin organoid culture (high clensit!, c ~ ~ l t u r e ) l'or .; weeks in n Trowell systern. During the first 48 h n sor t ing-o~~ptr ocess took place. i.e. liver and bloodforming cells met to form aggregates. In between mescncliynial cells were seen. Vessel-like cavities cleceloped. Electron microscopic inspection of the hepatocytes did not reveal any lesions of the cell organelles after 14 days in culture. As late as after a 3-week culture period niitochondrial s\~cllings ancl an increasecl number of autopliagic cacuole\ \\.ere ohse~.vccl. A rim of collagenous fibrils or fibrillar hun~llcs ancl granular matrix structul-es uas perceptible ;I\ earl! as after 7 days in culture. Immunofluoresccnce microscopic t e c h n i q~~eresv ealed collagen types 111. IV and V1 as well as laminin. nidogen. lieparansulfateproteoglycan and fibronectin in these areas. Thus, the composition of the matrix in this c u l t ~ ~ rsey stem corresponds (apart from the absence of collagen type 1 ) to tlie embryonic situation. Therefore. the organoid culture appears to be a n appropriate technicl~let o stuciy the l > c l i a ~ i oo~f~ lri epatocytes in \,itro. It is especially suited to clenionstrate the formation of matrix coliiponents in liver cells and their extracellular occurrence.
Autor/es principal/es: Elkasaby, A.
Xu, D.
Schroter-Kermanil, Chr.
Merker, H. J.
Forma parte de: Histology and histopathology
URI: http://hdl.handle.net/10201/18220
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 12
Derechos: info:eu-repo/semantics/openAccess
Aparece en las colecciones:Vol. 6, nº 2 (1991)



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