Scanning electron microscopic study of natural killer cell-mediated cytotoxicity

Abstract

The interaction between human natural killer (NK) celis and NK-susceptible target cells, as well as the mechanism involved in target cell lysis, were studied with scanning electron microscopy (SEM). Low density human peripheral blood lymphocytes, highly enriched with large granular lymphocytes (LGL), were used as effector cells, and K562-cells were used as NK-susceptible target ceiis. The surface features of LGLINK cells were examined under SEM. In the area of interaction, NWtarget-cell conjugates showed microvilli andor filipodia, and extensive areas of intercellular contact. In addition, the effector cells in some NWtargetcell conjugates were polarized toward the target cell. Changes in target cell surface features included loss of microvilli, large surface blebs and the appearance of small pore-like lesions on the cell membrane. Our findings show that target cell lysis occurred by apoptosis and plasma membrane lesions analogous to those seen during complement-mediated cytotoxicity.