Observations on cell kinetics and viability of a human melanoma cell line exposed to dicarboxylic acids in tissue culture

Abstract

Cultures of human nlelanonla cell line BOO08 \verc e-iposed to the disodium salts of arelaic acid (C,, 2Na), adipic acid (C,, 2Na) and dodecanediaic acid (C,, 2Na) at IO'M and 5 x 10-'M for 24 hrs. None of the diacid salts had a significant effect on growth rate or viability of the cells, at 10-'M for 24 hrs nor had C, 2Na any effect at 5 x 10'M. At 5 x 102M for 24 hrs, both C,, 2Na, and CIL2 Na had a significant effect in reducing both growth and viability. These effects were accompanied by morphological evidence of cell death, and swelling of mitochondria and accuni~~latioonf lipid droplets within cytoplasm of still biable cells.