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Title: An autoradiographic study of the uptake of tritiated proline by osteoblasts during hibernation
Issue Date: 1986
Publisher: Murcia : F. Hernández
ISSN: 0213-3911
Related subjects: CDU::6 - Ciencias aplicadas::61 - Medicina::611 - Anatomía
Keywords: Autoradiography
Abstract: Twenty-four LSH and LVG strain golden hamsters, Mesocricetus auratus, were used. Experimental animals were maintained at 5 C and allowed to hibernate. Control animals were kept at 27 C. Six animals (3 experimental, 3 control) were injected subcutaneously with I pCi of 'H-proline/gm body wt. (Spec. act. 3 Ci/mM) after hibernation lasting 12 hours, I day, 3 days, or 7 days. Animals were killed I hour after injection and autoradiographs were prepared from 5 Frn thick ilecalcified sections of femurs. A greater number of endosteal cells were labeled than periosteal cells and also exhibited a greater magnitude of labeling throughout the study. Differences between endosteal and periosteal cells both in percentage of cells labeled and magnitude of labeling were maximum in control animals and progressively decreased with increasing periods of hibernation. A reduction in synthesis of matrix proteins during the early period of hibernation was seen and was attributed to a significant reduction both in average cell activity and in the number of active cells during hibernation. The latter phenomenon apparently made a large contribution to the reduced matrical synthesis. 'H-proline uptake by osteoblasts probably retlects the reduced requirements of matrical synthesis during hibernation.
Primary author: Steinberg, B.
Singh, I.J.
Mitchell, O.G.
Published in: Histology and histopathology
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 6
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Vol. 1, nº 2 (1986)

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