Please use this identifier to cite or link to this item: http://hdl.handle.net/10201/17814

Title: Comparative cytospectrophotometry of Wright-stained erythroid cells of vertebrates
Issue Date: 1986
Publisher: Murcia : F. Hernández
ISSN: 0213-3911
Related subjects: CDU::6 - Ciencias aplicadas::61 - Medicina::611 - Anatomía
Keywords: Cytospectrophotometry
Erythroid cell
Abstract: Visible spectra of erythroid cells stained with Wright's method were studied microphotometrically. Most mature cells of vertebrates have the main peak at 520 nm (A 520) and accessory peaks at 410 nm and 600 nm (A410 and A600), respectively. To compare the absorption at 520nm against 410nm and 600 nm, the relative absorption spectra were determined and effectively shown as percentage ratios (RAs) to the main peak of A520. The A520 shifted to the shorter side of the wavelengths in birds and lower vertebrates. When the RA values of 410 and 600 nm were compared, it was observed that RA 410 of immature cells was less than that of mature cells (15%), and that immature cells of the lower RA 410 type were present in peripheral blood, as well as in bone marrow of vertebrates. Thus, photometric analysis of erythroid cells at RA 410 and RA 600 against A 520 seems to provide useful parameters for comparative studies on vertebrate erythroid cells. Six spectral types can be observed in vertebrate erythroid cells by these parameters
Primary author: Shigeyoshi Kamo
Hisashi Takeuchi
Masa-oki Yamada
Published in: Histology and histopathology
URI: http://hdl.handle.net/10201/17814
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 7
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Vol. 1, nº 2 (1986)

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