Por favor, use este identificador para citar o enlazar este ítem: https://doi.org/10.14670/HH-18-869

Título: Testicular cryopreservation: From technical aspects to practical applications
Fecha de publicación: 2025
Editorial: Universidad de Murcia, Departamento de Biologia Celular e Histiologia
Cita bibliográfica: Histology and Histopathology Vol. 40, nº07 (2025)
ISSN: 0213-3911
1699-5848
Materias relacionadas: CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología
Palabras clave: Spermatogenesis
Biobank
Cryogenics
Fertility restoration
Resumen: Testicular cryopreservation has been highlighted as a promising alternative for preserving male fertility and can be applied to restore spermatogenesis in prepubertal individuals or cancer patients, preserve biologically valuable genotypes, and in studies on reproductive physiology or toxicity of various substances. This review presents an analysis of the technical aspects and applications of testicular cryopreservation, examining the contributions of important studies in this area and discussing the different factors that can impact the efficiency of the technique. Testicular fragments can be obtained from living or dead individuals, at any age and reproductive stage, through orchiectomy or biopsy. Among the methods used for processing, slow freezing and vitrification in open or closed systems stand out. However, factors such as species, age, medium used, cryoprotectants, and cryopreservation method can influence the viability of the testis after heating. To obtain sperm, the testes can be cultured in vitro or in vivo and the recovered gametes applied in assisted reproduction techniques. However, in some species, mainly wild animals and humans, this is still a limitation to be overcome.
Autor/es principal/es: Pereira, Ana Glória
Matos, Tayná Moura
Albuquerque, Joana Letícia Cottin de
Silva, Andréia Maria da
Silva, Alexandre Rodrigues
URI: http://hdl.handle.net/10201/157041
DOI: https://doi.org/10.14670/HH-18-869
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 12
Derechos: info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Aparece en las colecciones:Vol.40, nº7 (2025)

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