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dc.contributor.authorGandía-Herrero, Fernando-
dc.contributor.authorGarcía-Carmona, Francisco-
dc.date.accessioned2025-07-02T06:52:53Z-
dc.date.available2025-07-02T06:52:53Z-
dc.date.created2011-11-24-
dc.date.issued2012-01-24-
dc.identifier.citationPlanta (2012) 236:91–100 ISSN: , Año de publicación: 2012 Volumen: 236 Número: 1 Páginas: 91-100 Tipo: Artículoes
dc.identifier.issnPrint.: 0032-0935-
dc.identifier.issnElectronic.: 1432-2048-
dc.identifier.urihttp://hdl.handle.net/10201/156846-
dc.description© Springer-Verlag 2012. This document is the Published version of a Published Work that appeared in final form in Planta. To access the final edited and published work see https://doi.org/10.1007/s00425-012-1593-2es
dc.description.abstractBetalains are water-soluble pigments with high antiradical capacity which bestow bright colors to flowers, fruits and other parts of most plants of the order Caryophyllales. The formation of the structural unit of all betalains, betalamic acid from the precursor amino acid 4,5-dihydroxyphenylalanine is catalyzed by the enzyme 4,5-DOPAextradiol-dioxygenase followed by intramolecular cyclization of the 4,5-secodopa intermediate. This paper describes the purification and the molecular and functional characterization of an active 4,5-DOPA-extradiol-dioxygenase from the best-known source of betalains—Beta vulgaris—after heterologous expression in Escherichia coli. The enzyme is a monomeric protein with a molecular mass of 32 kDa characterized by chromatography, electrophoresis and MALDITOF analysis. Enzyme kinetic properties are characterized in the production of betalamic acid, the structural, chromophoric and bioactive unit of plant pigment betalains.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.languageenges
dc.relationThis work was supported by the Ministerio de Ciencia e Innovación (MCINN, FEDER, Spain) (Project AGL2010-17938), and by Programa de Ayudas a Grupos de Excelencia de la Región de Murcia, of the Fundación Séneca, Agencia de Ciencia y Tecnología de la Región de Murcia (Plan Regional de Ciencia y Tecnología 2007/2010). F. Gandía-Herrero holds a contract with the ‘‘Programa Ramón y Cajal’’ (MICINN, FEDER, Spain). The authors are grateful to Sofía Jiménez for help in gel filtration analysis and to Alejandro Torrecillas (SAI, University of Murcia) for skillful technical assistance in MALDI-TOF experiments. Financiadores Fundación Séneca Spain Ministerio de Ciencia e Innovación Spain European Regional Development Fund European Union AGL2010-17938es
dc.rightsinfo:eu-repo/semantics/embargoedAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectBetalain biosynthesises
dc.subjectPlant pigmentes
dc.subjectBeta vulgarises
dc.subjectExtradioles
dc.subjectDioxygenasees
dc.subjectBetalamic acides
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísicaes
dc.titleCharacterization of recombinant Beta vulgaris 4,5-DOPA-extradiol-dioxygenase active in the biosynthesis of betalainses
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doihttps://doi.org/10.1007/s00425-012-1593-2-
dc.contributor.departmentBioquímica y Biología Molecular Aes
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