Comparing anticoagulant rodenticide exposure in barn owl (Tyto alba) and common kestrel (Falco tinnunculus): A biomonitoring study in an agricultural region of southeastern Spain

Abstract

Second-generation anticoagulant rodenticides (SGARs) are commonly used for rodent control, affecting various non-target wildlife species. Here, blood samples from common kestrels ( Falco tinnunculus, , n = 70 chicks) and barn owls ( Tyto alba, n = 54 chicks and 12 adults) from Southeastern Spain were analysed using HPLC-TQ. SGAR prevalence was 68.6% in kestrel chicks, 50% in barn owl chicks and 100% in adult barn owls, with multiple SGARs in both species. Prothrombin time analysis in barn owls revealed a positive correlation with blood Sigma SGARs, suggesting a potential adverse effect on coagulation. Analysis of variables potentially influencing SGAR prevalence indicated that, for kestrels, it was only related to the extent of artificial surface, showing no differences across study sites. In owlets, the highest prevalence occurred in the most urbanized study site, with human population density being a key factor. This study highlights species-specific differences in SGAR exposure, likely influenced by ecological traits. Barn owls probably encounter contaminated prey near anthropized areas, with widespread SGAR use and higher presence of target rodents. Conversely, kestrels, hunting a variety of prey often near human settlements, face consistently elevated exposure from multiple sources. Understanding these variations is crucial for effective conservation and minimizing SGAR impact on non-target wildlife.