Por favor, use este identificador para citar o enlazar este ítem: https://doi.org/10.1016/j.vetimm.2006.10.008

Título: Characterization of a murine model of intranasal infection suitable for testing vaccines against C. abortus
Fecha de publicación: 15-ene-2007
Editorial: Elsevier
Cita bibliográfica: Veterinary Immunology and Immunopathology, 2007, Vol. 115, Issues 1–2, pp. 76-86
ISSN: Print: 0165-2427
Electronic: 1873-2534
Palabras clave: Chlamydophila abortus
Mouse model
Adjuvants
Vaccine
Cellular immune response
Resumen: Mouse models have been widely used to test candidate vaccines against Chlamydophila abortus infection in mice. Although the induction of a systemic infection by endogenous or intraperitoneal inoculation is a useful tool for understanding the immune mechanism involved in the protection conferred by the vaccination, a different approach is necessary to understand other factors of the infection, such as mucosal immunity or the colonization of target organs. To test whether C. abortus intranasal model of infection in mice is a useful tool for testing vaccines in a first group of experiments mice, were infected intranasally with C. abortus to characterize the model of infection. When this model was used to test vaccines, two inactivated experimental vaccines, one of them adjuvated with QS-21 and another with aluminium hydroxide, and a live attenuated vaccine (strain 1B) were used. Non-vaccinated control mice died within the first 8 days, after displaying substantial loss of weight. Histologically, the mice showed lobar fibrinopurulent bronchointerstitial pneumonia. Prior immunization with QS-21 adjuvated vaccine or 1B vaccine presented mortality and the recipients showed a greater number of T cells in the lesions, especially CD8+ T cells, than the control mice and mice immunized with vaccine adjuvated with aluminium hydroxide. The results confirm that the C. abortus intranasal model of infection in mice is a useful tool for testing vaccines
Autor/es principal/es: Buendía, A. J.
Nicolás, L.
Ortega, N.
Gallego, M. C.
Martínez Cáceres, Carlos Manuel
Sánchez, J.
Caro, M. R.
Navarro, J. A.
Salinas, J.
Versión del editor: https://www.sciencedirect.com/science/article/pii/S0165242706002820
URI: http://hdl.handle.net/10201/148891
DOI: https://doi.org/10.1016/j.vetimm.2006.10.008
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 11
Derechos: info:eu-repo/semantics/embargoedAccess
Descripción: © 2006 Elsevier B.V. This document is the Published version of a Published Work that appeared in final form in Veterinary Immunology and Immunopathology. To access the final edited and published work see https://doi.org/10.1016/j.vetimm.2006.10.008
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