Por favor, use este identificador para citar o enlazar este ítem:
https://doi.org/10.3390/biology9090276
Twittear
Título: | Measurable cytokine concentrations in pig seminal plasma are modified by semen handling and storage |
Fecha de publicación: | 7-sep-2020 |
Editorial: | MDPI |
Cita bibliográfica: | Biology, 2019, Vol. 9 (9): 276 |
ISSN: | Electronic: 2079-7737 |
Palabras clave: | Seminal plasma Sorage Cytokines Pg |
Resumen: | Sample handling and storing are critical steps for the reliable measurement of circulating biomolecules in biological fluids. This study evaluates how cytokine measurements in pig seminal plasma (SP) vary depending on semen handling and SP storage. Thirteen cytokines (GM-CSF, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18 and TNFα) were measured using Luminex xMAP® technology in individual seminal plasma (SP) samples (n = 62) from healthy breeding boars. Three separate experiments explored the delay (2 h and 24 h) in SP collection after ejaculation (Experiment 1) and SP storage, either short-term (5 °C, -20 °C and -80 °C for 72 h, Experiment 2) or long-term (at -20 °C and -80 °C for two months, Experiment 3), before analysis. Levels in fresh SP-samples were used as baseline control values. Delays in SP harvesting of up to 24 h did not substantially impact SP cytokine measurements. Some cytokines showed instability in stored SP samples, mainly in long-term storage. Ideally, cytokines in pig SP should be measured in fresh samples harvested within 24 h after ejaculation. If storage of SP is imperative, storage conditions should be adjusted for each cytokine. |
Autor/es principal/es: | Padilla, Lorena Barranco, Isabel Parrilla, Inmaculada Lucas, Xiomara Rodríguez-Martínez, Heriberto Roca, Jordi |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Medicina y Cirugía Animal |
Versión del editor: | https://www.mdpi.com/2079-7737/9/9/276 |
URI: | http://hdl.handle.net/10201/143932 |
DOI: | https://doi.org/10.3390/biology9090276 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 16 |
Derechos: | info:eu-repo/semantics/openAccess Atribución 4.0 Internacional |
Descripción: | © 2020 by the authors. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/ This document is the Published version of a Published Work that appeared in final form in Biology. To access the final edited and published work see https://doi.org/10.3390/biology9090276 |
Aparece en las colecciones: | Artículos: Medicina y Cirugía Animal |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
biology-09-00276.pdf | 1,04 MB | Adobe PDF | Visualizar/Abrir |
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons