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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Pérez-Patiño, Cristina | - |
dc.contributor.author | Parrilla, Inmaculada | - |
dc.contributor.author | Li, Junwei | - |
dc.contributor.author | Barranco, Isabel | - |
dc.contributor.author | Martínez, Emilio A. | - |
dc.contributor.author | Rodríguez-Martínez, Heriberto | - |
dc.contributor.author | Roca, Jordi | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Medicina y Cirugía Animal | es |
dc.date.accessioned | 2024-09-13T07:13:21Z | - |
dc.date.available | 2024-09-13T07:13:21Z | - |
dc.date.issued | 2019-01 | - |
dc.identifier.citation | Molecular & Cellular Proteomics (MCP), 2019, Vol. 18 (1), pp. 41-50 | es |
dc.identifier.issn | Electronic: 1535-9484 | - |
dc.identifier.uri | http://hdl.handle.net/10201/143931 | - |
dc.description | © 2019 Pérez-Patiño et al. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/ This document is the Published version of a Published Work that appeared in final form in Molecular & Cellular Proteomics (MCP). To access the final edited and published work see https://doi.org/10.1074/mcp.RA118.000840 | - |
dc.description.abstract | Proteins are essential for sperm function, including their fertilizing capacity. Pig spermatozoa, emitted in well-defined ejaculate fractions, vary in their functionality, which could be related to different sperm protein composition. This study aimed (i) to update the porcine sperm proteome and (ii) to identify proteins differentially expressed in mature spermatozoa from cauda epididymis and those delivered in separate ejaculate fractions. Ejaculates from nine mature and fertile boars were manually collected in three separate portions: the first 10 ml of the sperm-rich ejaculate fraction (SRF), the rest of the SRF and the post-SRF. The contents of cauda epididymides of the boars were collected post-mortem by retrograde duct perfusion, generating four different semen sources for each boar. Following centrifugation, the resulting pellets of each semen source were initially pooled and later split to generate two technical replicates per source. The resulting eight sperm samples (two per semen source) were subjected to iTRAQ-based 2D-LC-MS/MS for protein identification and quantification. A total of 1,723 proteins were identified (974 of Sus scrofa taxonomy) and 1,602 of them were also quantified (960 of Sus scrofa taxonomy). After an ANOVA test, 32 Sus scrofa proteins showed quantitative differences (p < 0.01) among semen sources, which was particularly relevant for sperm functionality in the post-SRF. The present study showed that the proteome of boar spermatozoa is remodeled during ejaculation involving proteins clearly implicated in sperm function. The findings provide valuable groundwork for further studies focused on identifying protein biomarkers of sperm fertility. | es |
dc.format | application/pdf | es |
dc.format.extent | 11 | es |
dc.language | eng | es |
dc.publisher | Elsevier | - |
dc.relation | This study was supported by MINECO (Spain) and FEDER funds (EU) (AGL2015-69738-R), and Seneca Foundation Murcia, Spain (19892/GERM-15); FORSS (745971) and The Swedish Research Council FORMAS (2017-00946), Stockholm, Sweden. C. Perez Patin˜o, I. Barranco and J. Li were financially supported by Seneca Foundation (Murcia, Spain), MECD (Madrid, Spain) and the China Scholarship Council, respectively. | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Boar | es |
dc.subject | Clinical Proteomics | - |
dc.subject | Ejaculate | - |
dc.subject | Epididymis | - |
dc.subject | Protein Identification | - |
dc.subject | Quantification | - |
dc.subject | Tandem Mass Spectrometry | - |
dc.subject | iTRAQ | - |
dc.title | The proteome of pig spermatozoa Is remodeled during ejaculation | es |
dc.type | info:eu-repo/semantics/article | es |
dc.relation.publisherversion | https://www.mcponline.org/article/S1535-9476(20)34100-1/fulltext | es |
dc.identifier.doi | https://doi.org/10.1074/mcp.RA118.000840 | - |
Aparece en las colecciones: | Artículos: Medicina y Cirugía Animal |
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PIIS1535947620341001.pdf | 1,28 MB | Adobe PDF | Visualizar/Abrir |
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