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Título: | The proteome of frozen-thawed pig spermatozoa is dependent on the ejaculate fraction source |
Fecha de publicación: | 24-ene-2019 |
Editorial: | Nature Research |
Cita bibliográfica: | Scientific Reports, 2019, Vol. 9 (1): 705 |
ISSN: | Electronic: 2045-2322 |
Resumen: | The preservation of sperm functional parameters and fertility post-cryopreservation largely varies in the porcine, a species with a fractionated ejaculate. Although intrinsic individual differences have primarily been linked to this variation, differences in protein abundance among frozen-thawed (FT)-spermatozoa are far more relevant. This study, performed in two experiments, looked for proteomic quantitative differences between FT-sperm samples differing in post-thaw viability, motility, apoptosis, membrane lipid peroxidation and nuclear DNA fragmentation. The spermatozoa were either derived from the sperm-rich ejaculate fraction (SRF) or the entire ejaculate (Experiment 1) or from the first 10 mL of the SRF, the remaining SRF and the post-SRF (Experiment 2). Quantitative sperm proteomic differences were analysed using a LC-ESI-MS/MS-based SWATH approach. In Experiment 1, FT-spermatozoa from the SRF showed better preservation parameters than those from the entire ejaculate, with 26 Sus scrofa proteins with functional sperm relevance showing relative quantitative differences (FC ≥ 1.5) between sperm sources. In Experiment 2, FT-spermatozoa from the first 10 mL of the SRF and the remaining SRF were qualitatively better than those from the post-SRF, and 187 proteins showed relative quantitative differences among the three ejaculate sources. The results indicate that quantitative proteome differences are linked to sperm cryosurvival. |
Autor/es principal/es: | Pérez-Patiño, Cristina Li, Junwei Barranco, Isabel Martínez, Emilio A. Rodríguez-Martínez, Heriberto Roca, Jordi Parrilla, Inmaculada |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Medicina y Cirugía Animal |
Versión del editor: | https://www.nature.com/articles/s41598-018-36624-5 |
URI: | http://hdl.handle.net/10201/143917 |
DOI: | https://doi.org/10.1038/s41598-018-36624-5 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 13 |
Derechos: | info:eu-repo/semantics/openAccess Atribución 4.0 Internacional |
Descripción: | © The Author(s) 2019. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/ This document is the Published version of a Published Work that appeared in final form in Scientific Reports. To access the final edited and published work see https://doi.org/10.1038/s41598-018-36624-5 |
Aparece en las colecciones: | Artículos: Medicina y Cirugía Animal |
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