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Título: | Seminal plasma modulates miRNA expression by sow genital tract lining explants |
Fecha de publicación: | 19-jun-2020 |
Editorial: | MDPI |
Cita bibliográfica: | Biomolecules, 2020, Vol. 10 (6): 933 |
ISSN: | Electronic: 2218-273X |
Palabras clave: | Explants Female genital tract Immune response MiRNAs Mucosal tissue Seminal plasma Transcriptome |
Resumen: | The seminal plasma (SP) modulates the female reproductive immune environment after mating, and microRNAs (miRNAs) could participate in the process. Considering that the boar ejaculate is built by fractions differing in SP-composition, this study evaluated whether exposure of mucosal explants of the sow internal genital tract (uterus, utero-tubal junction and isthmus) to different SP-fractions changed the profile of explant-secreted miRNAs. Mucosal explants retrieved from oestrus sows (n = 3) were in vitro exposed to: Medium 199 (M199, Control) or M199 supplemented (1:40 v/v) with SP from the sperm-rich fraction (SRF), the post-SRF or the entire recomposed ejaculate, for 16 h. After, the explants were cultured in M199 for 24 h to finally collect the media for miRNA analyses using GeneChip miRNA 4.0 Array (Affymetrix). Fifteen differentially expressed (False Discovery Rate (FDR) < 0.05 and Fold-change ≥ 2) miRNAs (11 down- versus 4 up-regulated) were identified (the most in the media of uterine explants incubated with SP from post-SRF). Bioinformatics analysis identified that predicted target genes of dysregulated miRNAs, mainly miR-34b, miR-205, miR-4776-3p and miR-574-5p, were involved in functions and pathways related to immune response. In conclusion, SP is able to elicit changes in the miRNAs profile secreted by female genital tract, ultimately depending SP-composition. |
Autor/es principal/es: | Barranco, Isabel Padilla, Lorena Martínez, Cristina A. Álvarez-Rodríguez, Manuel Parrilla, Inmaculada Lucas, Xiomara Ferreira-Dias, Graça Yeste, Marc Rodríguez-Martínez, Heriberto Roca, Jordi |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Medicina y Cirugía Animal |
Versión del editor: | https://www.mdpi.com/2218-273X/10/6/933 |
URI: | http://hdl.handle.net/10201/143914 |
DOI: | https://doi.org/10.3390/biom10060933 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 22 |
Derechos: | info:eu-repo/semantics/openAccess Atribución 4.0 Internacional |
Descripción: | © 2020 by the authors.This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/ This document is the Published version of a Published Work that appeared in final form in Biomolecules. To access the final edited and published work see https://doi.org/10.3390/biom10060933 |
Aparece en las colecciones: | Artículos: Medicina y Cirugía Animal |
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