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Título: The Influence of a membrane environment on the structure and stability of a prokaryotic potassium channel, KcsA
Fecha de publicación: 26-sep-2005
Editorial: Elsevier
Cita bibliográfica: FEBS Letters, 2005, Vol. 579, Issue 23,pp. 5199-5204
ISSN: Print: 0014-5793
Electronic: 1873-3468
Palabras clave: Infrared spectroscopy
Amide I′ and II bands
Thermal denaturation
Resumen: The lack of a membrane environment in membrane protein crystals is considered one of the major limiting factors to fully imply X-ray structural data to explain functional properties of ion channels [Gulbis, J.M. and Doyle, D. (2004) Curr. Opin. Struct. Biol. 14, 440–446]. Here, we provide infrared spectroscopic evidence that the structure and stability of the potassium channel KcsA and its chymotryptic derivative 1–125 KcsA reconstituted into native-like membranes differ from those exhibited by these proteins in detergent solution, the latter taken as an approximation of the mixed detergent-protein crystal conditions.
Autor/es principal/es: Encinar Hidalgo, José Antonio
Molina Gallego, María Luisa
Poveda Larrosa, José Antonio
Barrera Olivares, Francisco Nicolás
Renart Pérez, María Lourdes
Fernández Carvajal, Asia María
González Ros, José Manuel
Versión del editor: https://www.sciencedirect.com/science/article/pii/S0014579305010380
URI: http://hdl.handle.net/10201/142859
DOI: https://doi.org/10.1016/j.febslet.2005.08.038
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 6
Derechos: info:eu-repo/semantics/embargoedAccess
Descripción: © 2005 Federation of European Biochemical Societies. This document is the Published version of a Published Work that appeared in final form in FEBS Letters. To access the final edited and published work see https://doi.org/10.1016/j.febslet.2005.08.038
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