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https://doi.org/10.1093/glycob/cwq220
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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Pérez Oliva, Ana B. | - |
dc.contributor.author | Martínez Esparza, M. | - |
dc.contributor.author | Vicente Fernández, José J. | - |
dc.contributor.author | Corral San Miguel, Rubén | - |
dc.contributor.author | García Peñarrubia, Pilar | - |
dc.contributor.author | Hernández Caselles, Trinidad | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Bioquímica y Biología Molecular B e Inmunología | es |
dc.date.accessioned | 2024-07-03T07:51:16Z | - |
dc.date.available | 2024-07-03T07:51:16Z | - |
dc.date.issued | 2011-01-28 | - |
dc.identifier.citation | Glycobiology, 2011, Vol. 21, Issue 6, pp. 757–770 | es |
dc.identifier.issn | Print: 0959-6658 | - |
dc.identifier.issn | Electronic: 1460-2423 | - |
dc.identifier.uri | http://hdl.handle.net/10201/142811 | - |
dc.description | © The Author 2011. This document is the Published version of a Published Work that appeared in final form in Glycobiology. To access the final edited and published work see https://doi.org/10.1093/glycob/cwq220 | - |
dc.description.abstract | We have tested the usefulness of several commercial anti-CD33 monoclonal antibodies (mAb) to determine the expression and localization of the two CD33 isoforms on several hematopoietic cell lines. The expression of the isoform CD33m, a CD33 transmembrane splice variant lacking the ligand-binding V immunoglobulin (Ig)-like domain, was detected by RT-polymerase chain reaction, western blot, confocal microscopy and flow cytometry on the membrane of several human cell types. CD33m was only detected by the anti-CD33 mAb HIM3-4 on the cell surface, whereas WM53, P67.6, 4D3, HIM3-4, WM54, D3HL60.251 or MY9 detected the CD33M isoform, indicating that HIM3-4 is the only mAb recognizing CD33 C(2) Ig domain. Accordingly, HIM3-4 binding to CD33 did not interfere with the binding of other antibodies against the CD33 V-domain. P67.6 mAb interfered with recognition by the rest of antibodies specific for the V domain. HIM3-4 staining could be increased after the sialidase treatment of all CD33(+) cells. However, this increase was stronger in activated T cells, suggesting a CD33 masking state in this cell population. Confocal microscopy analysis of CD33m HEK 293T-transfected cells revealed that this protein is expressed on the cell membrane and also detected in the Golgi compartment. CD33 is constitutively located outside the lipid raft domains, whereas cross-linked CD33 is highly recruited to this signaling platform. The unique ability of HIM3-4 mAb to detect the masking state of CD33 on different cell lineages makes it a good tool to improve the knowledge of the biological role of this sialic acid-binding Ig-like lectin. | es |
dc.format | application/pdf | es |
dc.format.extent | 14 | es |
dc.language | eng | es |
dc.publisher | Oxford University Press | es |
dc.relation | This work was supported by projects 03112/PI/05 and 11926/ PI/09 from Fundación Séneca (CARM, Spain). | es |
dc.rights | info:eu-repo/semantics/embargoedAccess | es |
dc.subject | Anti-CD33 mAb | es |
dc.subject | CD33 epitopes | es |
dc.subject | CD33m isoform | es |
dc.title | Epitope mapping, expression and post-translational modifications of two isoforms of CD33 (CD33M and CD33m) on lymphoid and myeloid human cells. | es |
dc.type | info:eu-repo/semantics/article | es |
dc.relation.publisherversion | https://academic.oup.com/glycob/article/21/6/757/1988816 | es |
dc.embargo.terms | SI | - |
dc.identifier.doi | https://doi.org/10.1093/glycob/cwq220 | - |
Aparece en las colecciones: | Artículos: Bioquímica y Biología Molecular "B" e Inmunología |
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Fichero | Descripción | Tamaño | Formato | |
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757_002 CD33 2011.pdf | 616,92 kB | Adobe PDF | ![]() Visualizar/Abrir Solicitar una copia |
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