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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Martínez-Esparza, M. | - |
dc.contributor.author | Sánchez-Fresneda, Ruth | - |
dc.contributor.author | Guirao-Abad, José P. | - |
dc.contributor.author | Maicas, Sergi | - |
dc.contributor.author | Valentín, Eulogio | - |
dc.contributor.author | Argüelles, Juan-Carlos | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Bioquímica y Biología Molecular B e Inmunología | es |
dc.date.accessioned | 2024-07-02T07:55:11Z | - |
dc.date.available | 2024-07-02T07:55:11Z | - |
dc.date.issued | 2015-10-31 | - |
dc.identifier.citation | Fungal Genetics and Biology 85 (2015) 45–57 | es |
dc.identifier.issn | Print: 1087-1845 | - |
dc.identifier.issn | Electronic: 1096-0937 | - |
dc.identifier.uri | http://hdl.handle.net/10201/142790 | - |
dc.description | © 2015 Elsevier Inc. All rights reserved. This document is the Published version of a Published Work that appeared in final form in Fungal Genetics and Biology. To access the final edited and published work see https://doi.org/10.1016/j.fgb.2015.10.007 | - |
dc.description.abstract | A double homozygous atc1Δ/atc1Δ/ntc1Δ/ntc1Δ mutant (atc1Δ/ntc1Δ KO) was constructed in the pathogen opportunistic yeast Candida parapsilosis by disruption of the two chromosomal alleles coding for NTC1 gene (encoding a neutral trehalase) in a Cpatc1Δ/atc1Δ background (atc1Δ KO strain, deficient in acid trehalase). The Cpatc1Δ/ntc1Δ KO mutant failed to counteract the inability of Cpatc1Δ cells to metabolize exogenous trehalose and showed a similar growth pattern on several monosaccharides and disaccharides. However, upon prolonged incubation in either rich medium (YPD) or nutrient-starved medium the viability of Cpatc1Δ cells exhibited a sensitive phenotype, which was augmented by further CpNTC1/NTC1 disruption. Furthermore, Cpatc1Δ/ntc1Δ KO cells had difficulty in resuming active growth in fresh YPD. This homozygous mutant also lacked any in vitro measurable trehalase activity, whether acid or neutral, suggesting that a single gene codes for each enzyme. By contrast, in Cpatc1Δ/ntc1Δ KO strain the resistance to oxidative and heat stress displayed by atc1Δ mutant was suppressed. Cpatc1Δ/ntc1Δ KO cells showed a significant decrease in virulence as well as in the capacity to form biofilms. These results point to a major role for acid trehalase (Atc1p) in the pathobiology of C. parapsilosis, whereas the activity of neutral trehalase can only partially counteract Atc1p deficiency. They also support the use of ATC1 and NTC1 genes as interesting antifungal targets. | es |
dc.format | application/pdf | es |
dc.format.extent | 13 | es |
dc.language | eng | es |
dc.publisher | Elsevier | es |
dc.relation | R. S-F. and J.P. G-A. received a partial fellowship from Vitalgaia España, S.L. This work has been partially supported by grant PI12/01797, integrated in the ‘‘Plan Nacional de I+D+I 2008-2011” and co-financed by ‘‘ISCIII-Subdirección General de Evaluación and the Fondo Europeo de Desarrollo Regional (FEDER)”. We are also indebted to the financial contract provided by Cespa, Servicios Auxiliares de Murcia, S.A. | es |
dc.rights | info:eu-repo/semantics/embargoedAccess | es |
dc.subject | Antioxidant enzymes | es |
dc.subject | Candida parapsilosis | es |
dc.subject | Gene knockout | es |
dc.subject | Stress resistance | es |
dc.subject | Trehalase | es |
dc.subject | Virulence factor | es |
dc.title | Homozygous deletion of ATC1 and NTC1 genes in Candida parapsilosis abolishes trehalase activity and affects cell growth, sugar metabolism, stress resistance, infectivity and biofilm formation | es |
dc.type | info:eu-repo/semantics/article | es |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S1087184515300402?via%3Dihub | - |
dc.embargo.terms | Si | - |
dc.identifier.doi | https://doi.org/10.1016/j.fgb.2015.10.007 | - |
Aparece en las colecciones: | Artículos: Bioquímica y Biología Molecular "B" e Inmunología |
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